Site information for: LasR in species Pseudomonas aeruginosa; genome accession: NC

Transcription Factor Binding Site Information

dbxref: CollecTF:EXPSITE_00008b00
Genome: Pseudomonas aeruginosa - NC_002516.2
TF: LasR [UniProtKB:P25084, view regulon]

Supporting Evidence

Binding site	Location	Publication	Experimental techniques used	Curation
GCGTCAGATTTCCTCG	+ [2453398, 2453413]	19682264	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. -	424

Regulated genes

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Gene	Locus tag	Description
pslA	PA2231	protein PslA
pslB	PA2232	protein PslB
pslC	PA2233	protein PslC
pslD	PA2234	protein PslD
pslE	PA2235	protein PslE
pslF	PA2236	protein PslF
pslG	PA2237	protein PslG
pslH	PA2238	protein PslH
pslI	PA2239	protein PslI
pslJ	PA2240	protein PslJ
pslK	PA2241	protein PslL
pslL	PA2242	hypothetical protein
PA2230	PA2230	hypothetical protein
PA2229	PA2229	hypothetical protein