Site information for: LasR in species Pseudomonas aeruginosa; genome accession: NC

Transcription Factor Binding Site Information

dbxref: CollecTF:EXPSITE_00008b20
Genome: Pseudomonas aeruginosa - NC_002516.2
TF: LasR [UniProtKB:P25084, view regulon]

Supporting Evidence

Binding site	Location	Publication	Experimental techniques used	Curation
TTTTCAGTTGGACTCG	+ [1257585, 1257600]	19682264	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. -	424

Regulated genes

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Gene	Locus tag	Description
PA1159	PA1159	cold-shock protein
PA1158	PA1158	two-component sensor
PA1160	PA1160	hypothetical protein
rrmA	PA1161	rRNA methyltransferase
dapE	PA1162	succinyl-diaminopimelate desuccinylase
ndvB	PA1163	NdvB