Site information for: RpoN in species Pseudomonas aeruginosa; genome accession: NC

Transcription Factor Binding Site Information

dbxref: CollecTF:EXPSITE_00021680
Genome: Pseudomonas aeruginosa - NC_002516.2
TF: RpoN [UniProtKB:P49988, view regulon]

Supporting Evidence

Binding site	Location	Publication	Experimental techniques used	Curation
CTGGCAGGTTGCCTGCC	- [3889872, 3889888]	12670680	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) Multiple sequence alignment (MSA) - ECO:0005556 MSA can be used to align promoter regions and highlight regions of conservation that may be indicative of transcription factor binding sites. -	705

Regulated genes

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Gene	Locus tag	Description
rhlI	PA3476	autoinducer synthesis protein RhlI