Curation Information

Publication
Regulation of catalase-peroxidase KatG is OxyR dependent and Fur independent in Caulobacter crescentus.;Italiani VC, da Silva Neto JF, Braz VS, Marques MV;Journal of bacteriology 2011 Apr; 193(7):1734-44 [21257767]
TF
OxyR [A0A0H3CCL0, view regulon]
Reported TF sp.
Caulobacter crescentus NA1000
Reported site sp.
Caulobacter crescentus NA1000
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

katG transcription start site was identified by primer extension analysis using mRNA from strain NA1000. katG-lacZ fusion demonstrated OxyR-dependent regulation of katG. EMSA confirmed that this regulation was direct. DNase I footprinting determined OxyR binding region in the katG promoter.

Transcription Factor Binding Sites


CCGGCCACGGAATGGTTTCCTCTATCCATCCGATAAGAACAATCAATTTTA
CCGGCCACGGAATGGTTTCCTCTATCCATCCGATAAGAACAATCAATTTTA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CCGGCCACGGAATGGTTTCCTCTATCCATCCGATAAGAACAATCAATTTTA katG
... ... katG
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - activator monomer