Curation Information

Publication
Identification and cell cycle control of a novel pilus system in Caulobacter crescentus.;Skerker JM, Shapiro L;The EMBO journal 2000 Jul 3; 19(13):3223-34 [10880436]
TF
CtrA [P0CAW8, view regulon]
Reported TF sp.
Caulobacter crescentus NA1000
Reported site sp.
Caulobacter crescentus NA1000
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

pilA transcription start site was determined by primer extension analysis. Visual sequence inspection of the pilA promoter revealed a CtrA binding motif overlapping -35 region. Beta-gal activity analysis determined that pilA expression was reduced to 17% of the wild-type C. crescentus NA1000 expression level in the ctrA mutant strain. DNase I footprinting identified that a CtrA~P but not unphosphorylated CtrA protected three regions containing CtrA binding motifs.

Transcription Factor Binding Sites


TGGTTAAGAACAAATAACG
GTAAATACAAATAAACCAA
TGGTTAAGAACAAATAACG
GTAAATACAAATAAACCAA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGGTTAAGAACAAATAACG pilA,
... ... pilA CCNA_03044 CCNA_03045 CCNA_03046
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Visual sequence inspection (nan) - activator not specified
GTAAATACAAATAAACCAA pilA,
... ... pilA CCNA_03044 CCNA_03045 CCNA_03046
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details Visual sequence inspection (nan) - activator not specified