Curation Information

Publication

Acid-induced activation of the urease promoters is mediated directly by the ArsRS two-component system of Helicobacter pylori.;Pflock M, Kennard S, Delany I, Scarlato V, Beier D;Infection and immunity 2005 Oct; 73(10):6437-45 [16177315]

TF

ArsR [O24973, view regulon]

Reported TF sp.

Helicobactor Pylori 26695

Reported site sp.

Helicobacter pylori G27

Created by

Elizabeth Cardosa

Curation notes

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Experimental Process

Primer extension experiments were performed on RNAs extracted from mutant and non-mutant strains of helicobactor pylori G27 to identify that ureA and ureI are regulated by ArsR. DNase footprinting results show that there is direct binding of ArsR~P. Primer extension assay and western blot were used to show expression of the urease genes.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.