Computational search of HrpL binding sites was validated by DNA array analysis or Northern blot analysis using DC3000 (WT) and hrpL (L–) mutants in hrp-inducing medium.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
Site sequence | Regulated genes | Gene diagram | Experimental techniques | TF function | TF type |
---|---|---|---|---|---|
TGGAACCAACTTGCACCTTCAACCACACA | hrpK1, |
|
activator | not specified | |
TGGAACTGAAATCGATGCTCGACCACTTA | hrpP, hrcU, |
|
activator | not specified | |
TGGAACCCGCTGGCATTGCATGCCACTCA | avrE1, |
|
activator | not specified | |
GGGAACCGGTCGCTGCGCTTTGCCACTCA | hrpW1, |
|
activator | not specified |