Curation Information

Publication
Identification of six type III effector genes with the PIP box in Xanthomonas campestris pv. campestris and five of them contribute individually to full pathogenicity.;Jiang W, Jiang BL, Xu RQ, Huang JD, Wei HY, Jiang GF, Cen WJ, Liu J, Ge YY, Li GH, Su LL, Hang XH, Tang DJ, Lu GT, Feng JX, He YQ, Tang JL;Molecular plant-microbe interactions : MPMI 2009 Nov; 22(11):1401-11 [19810809]
TF
HrpX [A0A0H2X9M1, view regulon]
Reported TF sp.
Xanthomonas campestris pv. campestris str. 8004
Reported site sp.
Xanthomonas campestris pv. campestris str. 8004
Created by
Erill Lab
Curation notes
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Experimental Process

A genome-wide computational search for pip-boxes was performed, and the results validated by gus-reporter assays with hrpX-deletion mutant in the promoters of six genes that had been shown to induce HR response in plants through translocation assays.

Transcription Factor Binding Sites


TTCGGAGCAATAACCATCCGTTCGC
TTCGCACATGAAAATAAAGGTTCGC
TTCGCTAGCTCGCGCAGAGTTTCGC
TTCGTGTCGTTGCAACGCCCTTCGC
TTCGTCGTCCCGGTCAATTCTTCGG
TTCGCCATACCGATGAAGTCTTCGC
TTCGGAGCAATAACCATCCGTTCGC
TTCGCACATGAAAATAAAGGTTCGC
TTCGCTAGCTCGCGCAGAGTTTCGC
TTCGTGTCGTTGCAACGCCCTTCGC
TTCGTCGTCCCGGTCAATTCTTCGG
TTCGCCATACCGATGAAGTCTTCGC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TTCGGAGCAATAACCATCCGTTCGC XC_2994,
... ... XC_2994 XC_2993 XC_2995 XC_2996 XC_2997
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details GUS reporter gene assay (ECO:0005641) - activator not specified
TTCGCACATGAAAATAAAGGTTCGC XC_2995,
... ... XC_2995 XC_2994 XC_2993 XC_2996 XC_2997
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details GUS reporter gene assay (ECO:0005641) - activator not specified
TTCGCTAGCTCGCGCAGAGTTTCGC XC_3160
... ... XC_3160
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details GUS reporter gene assay (ECO:0005641) - activator not specified
TTCGTGTCGTTGCAACGCCCTTCGC XC_3177,
... ... XC_3177 XC_3176
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details GUS reporter gene assay (ECO:0005641) - activator not specified
TTCGTCGTCCCGGTCAATTCTTCGG XC_3802,
... ... XC_3802 XC_3803 XC_3804
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details GUS reporter gene assay (ECO:0005641) - activator not specified
TTCGCCATACCGATGAAGTCTTCGC XC_4273,
... ... XC_4273 XC_4274
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details GUS reporter gene assay (ECO:0005641) - activator not specified