Curation Information

Publication
Widespread distribution of a lexA-regulated DNA damage-inducible multiple gene cassette in the Proteobacteria phylum.;Abella M, Erill I, Jara M, Mazón G, Campoy S, Barbé J;Molecular microbiology 2004 Oct; 54(1):212-22 [15458417]
TF
LexA [P37452, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Erill Lab
Curation notes
-

Experimental Process

Binding assessed through EMSA. Mit-C induction by RT-PCR.

Transcription Factor Binding Sites


CACTGGATAAAAACACAGAG
CACTGTATATAATCCCAGTC
CACTGGATAAAAACACAGAG
CACTGTATATAATCCCAGTC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CACTGGATAAAAACACAGAG lexA, PA3008,
... ... lexA PA3008 psrA PA3009
Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor dimer
CACTGTATATAATCCCAGTC lexA, PA3008,
... ... lexA PA3008 psrA PA3009
Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor dimer