Curation Information

Publication
Transcriptional regulation of opaR, qrr2-4 and aphA by the master quorum-sensing regulator OpaR in Vibrio parahaemolyticus.;Zhang Y, Qiu Y, Tan Y, Guo Z, Yang R, Zhou D;PloS one 2012; 7(4):e34622 [22506036]
TF
OpaR [Q79YV4, view regulon]
Reported TF sp.
Vibrio parahaemolyticus RIMD 2210633
Reported site sp.
Vibrio parahaemolyticus RIMD 2210633
Created by
Elliot White
Curation notes
Targets are qrr2-4, which are sRNAs, and are presently not annotated in the genome.

Experimental Process

Experimenters used EMSAs and DNase protection assays to demonstrate binding, and used comparisons between the primer extension assays of wt and ∆OpaR to determine regulatory impact

Transcription Factor Binding Sites


GACTAACTCAATTGTTAATA
TGTTTATTAATCAATCATTA
TGCTGAGAAAGTGATTAGTA
GACTAACTCAATTGTTAATA
TGTTTATTAATCAATCATTA
TGCTGAGAAAGTGATTAGTA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
GACTAACTCAATTGTTAATA
... ... VPA1623 VPA1622 VPA1624
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Primer Extension assay (ECO:0005657) - activator dimer
TGTTTATTAATCAATCATTA
... ... VPA1240 VPA1241
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Primer Extension assay (ECO:0005657) - activator dimer
TGCTGAGAAAGTGATTAGTA
... ... VPA0199 VPA0200
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Primer Extension assay (ECO:0005657) - activator dimer