Curation Information

AmrZ modulates Pseudomonas aeruginosa biofilm architecture by directly repressing transcription of the psl operon.;Jones CJ, Ryder CR, Mann EE, Wozniak DJ;Journal of bacteriology 2013 Apr; 195(8):1637-44 [23354748]
AmrZ [G3XCY4, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Kathryn Cronise
Curation notes

Experimental Process

A Western and ELISA were each done to confirm expression, which was also verified by qRT-PCR. A series of EMSAs were performed to localize a binding site. An sequence alignment was performed to come up with a putative binding site, which was then confirmed to bind via EMSA.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
GCCACTATCGACGAA pslA, pslB, pslC, pslD, pslE, pslF, pslG, pslH, pslI, pslJ, pslK,
... ... pslA pslB pslC pslD pslE pslF pslG pslH pslI pslJ pslK PA2230 PA2229
Experimental technique details ELISA (ECO:0005581) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Western blot (quantitative) expression analysis (ECO:0000279) - repressor not specified