Curation Information

Publication
The zinc-responsive regulon of Neisseria meningitidis comprises 17 genes under control of a Zur element.;Pawlik MC, Hubert K, Joseph B, Claus H, Schoen C, Vogel U;Journal of bacteriology 2012 Dec; 194(23):6594-603 [23043002]
TF
Zur [Q9JZ70, view regulon]
Reported TF sp.
Neisseria meningitidis MC58
Reported site sp.
Neisseria meningitidis MC58
Created by
Dinara Sagitova
Curation notes
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Experimental Process

Zur-regulated genes were identified by DNA microarray analysis. Zur-mediated expression regulation was confirmed by qRT-PCR experiments. Promoter regions of the identified genes were scanned for the presence of a Zur box by alignments using ClustalW. Also, upstream regions were searched for conserved motifs with the GIBBS Motif Sampler. EMSAs were performed to confirm that Zur binds to in silico - predicted motifs.

Transcription Factor Binding Sites


TGTTATAACATAACA
TGTTATATAATAACA
TGTTATAACATAACA
TGTTATATAATAACA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGTTATAACATAACA NMB1475,
... ... NMB1475 NMB1474
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified
TGTTATATAATAACA NMB0964,
... ... NMB0964 NMB0965
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified