Curation Information

Publication
The virulence activator AphA links quorum sensing to pathogenesis and physiology in Vibrio cholerae by repressing the expression of a penicillin amidase gene on the small chromosome.;Kovacikova G, Lin W, Skorupski K;Journal of bacteriology 2003 Aug; 185(16):4825-36 [12897002]
TF
AphA [H9L4T0, view regulon]
Reported TF sp.
Vibrio cholerae O395
Reported site sp.
Vibrio cholerae O395
Created by
Erill Lab
Curation notes
-

Experimental Process

Based on previous DNAse foot print. Site directed mutagenesis on site, with both beta-gal and EMSA to verify activity/binding

Transcription Factor Binding Sites


TATGCAATCGAGTTCTCA
TATGCAATCGAGTTCTCA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TATGCAATCGAGTTCTCA tcpP,
... ... tcpP tcpI tcpH
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator dimer