Curation Information

Publication
RovA is autoregulated and antagonizes H-NS-mediated silencing of invasin and rovA expression in Yersinia pseudotuberculosis.;Heroven AK, Nagel G, Tran HJ, Parr S, Dersch P;Molecular microbiology 2004 Aug; 53(3):871-88 [15255899]
TF
H-NS [Q6VQX1, view regulon]
Reported TF sp.
Yersinia pseudotuberculosis YPIII
Reported site sp.
Yersinia pseudotuberculosis YPIII
Created by
Erill Lab
Curation notes
-

Experimental Process

EMSA to validate multiple binding sites. Beta-gal assays with deletions in promoter region show H-NS-mediated induction. DNAse footprinting with purified H-NS.

Transcription Factor Binding Sites


AATTATTTAAGGTATATATTGTCTATAGCAATTATATTATTTGAATTAATTTACATCCATTAATTATATT
GGTATAGACTGTTTTATTATATATTTTGAATATTTTTA
TTTTTAAAAAAGTGCTAT
TAAATTAGAAAACAAGTTTCACTTTCAAC
AATTATTTAAGGTATATATTGTCTATAGCAATTATATTATTTGAATTAATTTACATCCATTAATTATATT
GGTATAGACTGTTTTATTATATATTTTGAATATTTTTA
TTTTTAAAAAAGTGCTAT
TAAATTAGAAAACAAGTTTCACTTTCAAC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type