Curation Information

Publication
Discovery of Fur binding site clusters in Escherichia coli by information theory models.;Chen Z, Lewis KA, Shultzaberger RK, Lyakhov IG, Zheng M, Doan B, Storz G, Schneider TD;Nucleic acids research 2007; 35(20):6762-77 [17921503]
TF
Fur [P0A9A9, view regulon]
Reported TF sp.
Escherichia coli str. K-12 substr. MG1655
Reported site sp.
Escherichia coli str. K-12 substr. MG1655
Created by
Urooq Aqeel
Curation notes
-

Experimental Process

PSSM used to locate the site. EMSA used to verify the binding site. Fur binding site was confirmed through DNAse I footprinting.

Transcription Factor Binding Sites


CTTGCAAATGATAAAAATTATCATATGATAT
CTTGCAAATGATAAAAATTATCATATGATAT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CTTGCAAATGATAAAAATTATCATATGATAT
... ... fhuF bglJ yjjQ yjjZ leuV leuP leuQ
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer