Curation Information

Publication
Reconstruction of the evolutionary history of the LexA-binding sequence.;Mazón G, Erill I, Campoy S, Cortés P, Forano E, Barbé J;Microbiology (Reading, England) 2004 Nov; 150(Pt 11):3783-95 [15528664]
TF
LexA [P0DN68, view regulon]
Reported TF sp.
Fibrobacter succinogenes subsp. succinogenes S85
Reported site sp.
Fibrobacter succinogenes subsp. succinogenes S85
Created by
Erill Lab
Curation notes
-

Experimental Process

Binding to promoter initially validated by EMSA. Site further identified with DNAse footprint and fully characterized by serial site-directed mutagenesis.

Transcription Factor Binding Sites


TTGCCCAGTTGTGCAT
TTGCCCAGTTGTGCAT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TTGCCCAGTTGTGCAT
... ... Fisuc_R0065 Fisuc_2819 Fisuc_2820 Fisuc_2821 Fisuc_2822
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor dimer