Curation Information

Publication
Identification of AlgR-regulated genes in Pseudomonas aeruginosa by use of microarray analysis.;Lizewski SE, Schurr JR, Jackson DW, Frisk A, Carterson AJ, Schurr MJ;Journal of bacteriology 2004 Sep; 186(17):5672-84 [15317771]
TF
AlgR [P26275, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Erill Lab
Curation notes
-

Experimental Process

Differentially expressed genes identified by DNA-array. Putative sites identified by MSA with known (algD) promoter sites. Function of site shown by restoration of twitching motility with motility plasmid on a algR mutant.

Transcription Factor Binding Sites


CCGTCGGTC
CCGTTGGCC
CCGTTTGGC
CCGTCGGTC
CCGTTGGCC
CCGTTTGGC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CCGTCGGTC pilY1, pilX, pilW, pilV, fimU, fimT, pilY2,
... ... pilY1 pilX pilW pilV fimU fimT pilY2 PA4548 pilR pilS pilE lytB PA4558 lspA ileS ribF PA4562
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - activator dimer
CCGTTGGCC fimT, fimU, pilV, pilW, pilX, pilY1, pilY2,
... ... fimT fimU pilV pilW pilX pilY1 pilY2 PA4548 pilR pilS pilE lytB PA4558 lspA ileS ribF PA4562
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - activator dimer
CCGTTTGGC fimT, fimU, pilV, pilW, pilX, pilY1, pilY2,
... ... fimT fimU pilV pilW pilX pilY1 pilY2 PA4548 pilR pilS pilE lytB PA4558 lspA ileS ribF PA4562
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - activator dimer