Curation Information

Publication
Identification of AlgR-regulated genes in Pseudomonas aeruginosa by use of microarray analysis.;Lizewski SE, Schurr JR, Jackson DW, Frisk A, Carterson AJ, Schurr MJ;Journal of bacteriology 2004 Sep; 186(17):5672-84 [15317771]
TF
AlgR [P26275, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Erill Lab
Curation notes
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Experimental Process

Differentially expressed genes identified by DNA-array. Putative sites identified by MSA with known (algD) promoter sites. Site function verified by S1 protection assay and observation of HCN production on algR mutant and w-t.

Transcription Factor Binding Sites


CCGTCGTTC
CCGTGGGCC
CCGTCGTTC
CCGTGGGCC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CCGTCGTTC hcnA, hcnB, hcnC,
... ... hcnA hcnB hcnC PA2192
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details S1 nuclease protection (ECO:0005666) - repressor dimer
CCGTGGGCC hcnC, hcnB, hcnA,
... ... hcnC hcnB hcnA PA2196 PA2197 PA2198 PA2199 PA2200 PA2201 PA2202 PA2203 PA2204
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details S1 nuclease protection (ECO:0005666) - repressor dimer