Curation Information

Publication
Expression of canonical SOS genes is not under LexA repression in Bdellovibrio bacteriovorus.;Campoy S, Salvador N, Cortés P, Erill I, Barbé J;Journal of bacteriology 2005 Aug; 187(15):5367-75 [16030231]
TF
LexA [Q6MHM9, view regulon]
Reported TF sp.
Bdellovibrio bacteriovorus HD100
Reported site sp.
Bdellovibrio bacteriovorus HD100
Created by
Erill Lab
Curation notes
-

Experimental Process

EMSA was performed to validate binding of purified LexA upstream of the lexA promoter. DNAse footprint was used to specify site, mutagenesis to precisely define it. RT-PCR was used to confirm expression induced with mitomycin C

Transcription Factor Binding Sites


ATTTACACTGTAAGT
ATTTACACTGTAAGT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
ATTTACACTGTAAGT lexA,
... ... lexA gspD Bd3509
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor dimer