Curation Information

Publication

Repression of Clostridium difficile toxin gene expression by CodY.;Dineen SS, Villapakkam AC, Nordman JT, Sonenshein AL;Molecular microbiology 2007 Oct; 66(1):206-19 [17725558]

TF

CodY [Q18BE1, view regulon]

Reported TF sp.

Clostridium difficile 630

Reported site sp.

Clostridium difficile 630

Created by

Dinara Sagitova

Curation notes

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Experimental Process

RT-PCR analysis was done to verify that CodY regulates tcdR gene expression. EMSA showed that CodY directly interacts with its target promoter. DNase I footprinting assay was used to identify the specific binding sites for CodY.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.