Curation Information

Publication
PcaR-mediated activation and repression of pca genes from Pseudomonas putida are propagated by its binding to both the -35 and the -10 promoter elements.;Guo Z, Houghton JE;Molecular microbiology 1999 Apr; 32(2):253-63 [10231483]
TF
PcaR [Q88N41, view regulon]
Reported TF sp.
Pseudomonas putida PRS1
Reported site sp.
Pseudomonas putida PRS1
Created by
Erill Lab
Curation notes
-

Experimental Process

Binding to specific regions of the pca promoter genes first determined by EMSA with multiple-sized fragments, then confirmed and refined by DNAse footprinting. Site further defined by premethylation interference.

Transcription Factor Binding Sites


GTTCGATAATCGCAC
GTTCGATAATCGCAC
GCTCGCACATCGCAC
GTTCGATAATCGCAC
GTTCGATAATCGCAC
GCTCGCAGATCGCAC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
GTTCGATAATCGCAC
... ... PP_1374 pcaR
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Premethylation interference footprinting (ECO:0005656) - repressor dimer
GTTCGATAATCGCAC
... ... PP_3950 pcaI pcaJ
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Premethylation interference footprinting (ECO:0005656) - repressor dimer
GCTCGCAGATCGCAC
... ... PP_3950 pcaI pcaJ
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Premethylation interference footprinting (ECO:0005656) - repressor dimer