A HapR EMSA of AphA promoter fragments showed that HapR binds between -82 and -56. This result was confirmed via DNase I footprinting, which showed HapR protecting -83 -> -58/-85 -> -60 (top/bottom strand). Within this region, the authors found a 7 bp inverted repeat (with 2 mismatches) that could serve as the HapR binding site.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|