Curation Information

Publication

Regulation of virulence gene expression in Vibrio cholerae by quorum sensing: HapR functions at the aphA promoter.;Kovacikova G, Skorupski K;Molecular microbiology 2002 Nov; 46(4):1135-47 [12421317]

TF

HapR [A0A0H3Q915, view regulon]

Reported TF sp.

Vibrio cholerae C6706

Reported site sp.

Vibrio cholerae C6706

Created by

Elliot White

Curation notes

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Experimental Process

A HapR EMSA of AphA promoter fragments showed that HapR binds between -82 and -56. This result was confirmed via DNase I footprinting, which showed HapR protecting -83 -> -58/-85 -> -60 (top/bottom strand). Within this region, the authors found a 7 bp inverted repeat (with 2 mismatches) that could serve as the HapR binding site.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.