Curation Information

Publication: FvbA is required for vibriobactin utilization in Pseudomonas aeruginosa.;Elias S, Degtyar E, Banin E;Microbiology (Reading, England) 2011 Jul; 157(Pt 7):2172-80 [21546589]
TF: Fur [Q03456, view regulon]
Reported TF sp.: Pseudomonas aeruginosa PAO1
Reported site sp.: Pseudomonas aeruginosa PAO1
Created by: Dinara Sagitova
Curation notes: -

Experimental Process

Functionality of a previously predicted Fur box in the fvbA promoter region was confirmed by FURTA. qPCR validated Fur-mediated repression of the fvbA promoter.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

CCAAATGGGAATGACTCTC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type
CCAAATGGGAATGACTCTC	PA4156,		Experimental technique details FURTA (ECO:0005633) FURTA - ECO:0005633 A technique used to locate and isolate Fur sites via cloning. The genome of a target organism is digested with restriction enzymes, and ligated into high copy plasmids to create a plasmid library, which is then cloned into a cell line with a fur-repressed reporter. In cells where the introduced high copy plasmid contains a fur site, fur will be titrated away from it's binding site on the reporter (and throughout the genome), marking the colony. The cell can then be isolated, the plasmid extracted and sequenced for further study. - Experimental technique details qPCR [quantitative real-time] (ECO:0005660) qPCR [quantitative real-time] - ECO:0005660 Quantitative PCR (or quantitative real time polymerase chain reaction) is a modification of the conventional PCR reaction used to amplify and simultaneously quantify a targeted DNA molecule. In its more standard incarnation, a inactive fluorescent dye is added to the PCR mix. This dye is activated upon binding double-stranded DNA; as the PCR iterates, there is more and more dsDNA and therefore more fluorescence intensity. -	repressor	not specified