Curation Information

Publication
Fur controls iron homeostasis and oxidative stress defense in the oligotrophic alpha-proteobacterium Caulobacter crescentus.;da Silva Neto JF, Braz VS, Italiani VC, Marques MV;Nucleic acids research 2009 Aug; 37(14):4812-25 [19520766]
TF
Fur [A0A0H3C2M1, view regulon]
Reported TF sp.
Caulobacter crescentus NA1000
Reported site sp.
Caulobacter crescentus NA1000
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

Putative Fur-binding sites in the genome of C. crescentus were detected by using a position weight matrix approach. EMSAs validated that Fur binds to the identified promoters. LacZ reporter assays validated Fur-mediated expression regulation.

Transcription Factor Binding Sites


ATTGCGAGTGACACTCAGA
ATTGCGACGCACTCGCAAT
ACTGATAATCGTTCGCAAT
ATTGCGAGTGACACTCAGA
ATTGCGACGCACTCGCAAT
ACTGATAATCGTTCGCAAT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
ATTGCGAGTGACACTCAGA CC_0139,
... ... CC_0139 CC_0138 CC_0137 CC_0136 CC_0135 CC_0134 CC_0140 CC_0141 CC_0142 CC_0143 CC_0144 CC_0145 CC_0146 CC_0147 CC_0148 CC_0149 CC_0150 CC_0151 CC_0152 hrcA CC_0154 CC_0155
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - repressor not specified
ATTGCGACGCACTCGCAAT CC_2928,
... ... CC_2928 CC_2927 CC_2926 CC_2925 CC_2924 CC_2929 CC_2930 CC_2931
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - repressor not specified
ACTGATAATCGTTCGCAAT CC_0711, CC_0712,
... ... CC_0711 CC_0712 CC_0710 CC_0709 CC_0708 CC_0707
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - repressor not specified