Curation Information

Publication
Cohabitation of two different lexA regulons in Pseudomonas putida.;Abella M, Campoy S, Erill I, Rojo F, Barbé J;Journal of bacteriology 2007 Dec; 189(24):8855-62 [17933893]
TF
LexA [P59479, view regulon]
Reported TF sp.
Pseudomonas putida KT2440
Reported site sp.
Pseudomonas putida KT2440
Created by
Erill Lab
Curation notes
-

Experimental Process

Array to detect genes. EMSA to validate binding. Mutant for LexA1/2 and RT-PCR to verify expression.

Transcription Factor Binding Sites


GTACAAATGTGC
GTACAAATGTGC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
GTACAAATGTGC PP_3901,
... ... PP_3901 hicB-2 hicA-2
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor dimer