Curation Information

Publication
ChIP analysis unravels an exceptionally wide distribution of DNA binding sites for the NtcA transcription factor in a heterocyst-forming cyanobacterium.;Picossi S, Flores E, Herrero A;BMC genomics 2014 Jan 13; 15(.):22 [24417914]
TF
NtcA [P0A4U6, view regulon]
Reported TF sp.
Nostoc sp. PCC 7120
Reported site sp.
Nostoc sp. PCC 7120
Created by
Erill Lab
Curation notes
-
External databases
NCBI GEO (GSE51865) http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE51865.

Experimental Process

ChIP seq with NtcA antibody was performed on Anabaena sp. PCC 7120 cells in a N-depleted medium. CysFinder was applied for motif discovery in the whole dataset.

ChIP assay conditions
Wild-type Anabaena sp. PCC 7120 cells growing in bubbled cultures with ammonium as the N source were subjected to incubation in a combined N-depleted medium for 3 hours, after which the cultures were treated with formaldehyde to fix the proteins bound to DNA. After cell lysis and DNA fragmentation, the extracts were treated with an anti-NtcA antibody to specifically immunoprecipitate the NtcA-bound DNA. The immunoprecipitated material was then incubated at 65ºC to reverse the crosslinking, and the DNA was isolated. A sample of total DNA was also isolated prior to anti-NtcA treatment of the extracts to serve as the control input sample. Cells of Anabaena sp. (also known as Nostoc sp.) strain PCC 7120 growing exponentially (3-5 μg Chl/ml) in the light (75 μE·m-2·s-1) at 30°C in BG110 medium supplemented with 10 mM NaHCO3 (referred to as BG110C) containing 6 mM NH4Cl and 12 mM TES and bubbled with a mixture of air + 1% CO2 were collected, washed with BG110C, resuspended in BG110C, and incubated in the same conditions for 3 h. Formaldehyde was then added to the cultures to a final concentration of 1%, and the cultures were incubated for 15 min (no aeration, occasional shaking). Glycine was added at 125 mM final concentration and the incubation was continued for 5 min to stop the fixing reaction. The cells were then filtered, washed with cold TBS (20 mM Tris–HCl, pH 7.4, 140 mM NaCl) and collected in tubes (25 ml of culture per tube). The pellets were frozen in liquid nitrogen and stored at -20°C until used.
ChIP notes
Cells corresponding to about 150 ml of culture (6 tubes) were used for each ChIP experiment. Pellets corresponding to about 25 ml of culture were resuspended in 500 μl of lysis buffer (50 mM HEPES/KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, supplemented with Mini EDTA-free protease inhibitor cocktail [Roche]). Cells were supplemented with 150 μl of glass beads (acid-washed, 425-600 μm [Sigma]) and broken in a multivortexer at 2000 rpm for 1 h at 4°C. The cell lysates were collected by centrifugation and the extracts were subjected to sonication to shear the DNA into about 200-bp fragments (40 cycles of 10s, 30s ice, 10% amplitude, in a Branson Digital Sonifier). After centrifugation to eliminate cell debris, the whole-cell extracts were stored at -20°C or immediately used for immunoprecipitation. Immunoprecipitation of DNA was carried out as described in Hanaoka and Tanaka, with some modifications. Whole-cell extracts were prepared at 4 mg/ml of total protein with lysis buffer (in 500 μl total volume). A 50-μl sample was taken as the input sample, and the extracts were pre-treated with 0.6 mg of lysis-buffer-equilibrated Dynabeads Protein G (Invitrogen) (to avoid non-specific binding of DNA to the Protein G). Anti-NtcA antibody (or H2O for the mock sample) was added and incubated at 4°C with rotation overnight. The extracts were treated with 0.6 mg of Dynabeads Protein G for 2 h at 4°C with rotation. The Dynabeads were washed twice with 1.5 ml of lysis buffer (5 min, rotation), and once with 1.5 ml each buffer 1 (lysis buffer containing 500 mM NaCl), buffer 2 (10 mM Tris–HCl, pH 8, 250 mM LiCl, 0.5% NP-40, 1 mM EDTA), and buffer 3 (10 mM Tris–HCl, pH 7.5, 1 mM EDTA, 50 mM NaCl). The Dynabeads were resuspended in a solution of DNase-free RNase A (0.2 μg/μl in TE), incubated for 30 min at 37°C, and washed with 1.5 ml wash buffer 3. To elute the immunoprecipitated material, the Dynabeads were resuspended in 50 μl of elution buffer (50 mM Tris–HCl, pH 7.5, 10 mM EDTA, 1% SDS) and incubated at 65°C for 30 min. The elution step was repeated once and the two eluates were combined. For crosslinking reversion, the eluted material was incubated at 65°C for 5 h. The input sample was processed in parallel (Tris–HCl, pH 7.5, EDTA and SDS was added to reach the same concentration as in the ChIP sample). To eliminate proteins, Proteinase K was added at 0.4 μg/μl (final concentration) and the mixture was incubated for 1 h at 55°C. DNA was purified by phenol/chloroform/isoamyl alcohol extraction (25:24:1) followed by two extractions with chloroform/isoamyl alcohol (24:1). DNA was ethanol-precipitated using ammonium acetate and glycogen, and the pellet was washed twice with 70% ethanol, air-dried and resuspended in 25 μl purified H2O. For ChIP-Seq DNA samples, this protocol was repeated three times using cells from independent inductions, and the resulting DNA was mixed together and concentrated to 25 μl. Input and ChIP DNA samples were sent for sequencing at the Functional Genomics Core Facility of the Institute for Research in Biomedicine, Barcelona (Spain) (Herbert Auer). Next generation sequencing was carried out using Illumina’s sequencing technology. ChIP DNA Sample Prep Kit (Illumina) was used for library preparation. Libraries were loaded at 8 pM concentration into the flow cell using the Cluster Station running recipe V7 with the Single-Read Cluster Generation Kit v4 (all Illumina). The flow cell was loaded into the Genome Analyzer II and samples were sequenced for 120 nucleotides from a single end using the Sequencing Kit v5 and recipe v8 (all Illumina). Manufacturer’s recommendations were strictly followed. Illumina sequencing data were pre-processed with the standard Illumina pipeline version 1.5 and sequences were aligned to the Anabaena sp. PCC 7120 genome (http://genome.microbedb.jp/cyanobase/Anabaena webcite) with the Bowtie software 0.12.5. The percentage of reads mapped to the genome was 92.3% for the Input sample (HQ reads: 30,192,934, 64.9% of total) and 94.2% for the ChIP sample (HQ reads: 31,352,138, 68.5% of total). The analysis of the results was carried out using the Triform algorithm method (Karl Kornacker). For detected double-strand peak regions, the peak locations were reported as the averages of the forward and reverse peak locations; the z-scores were calculated according to equations (4) - (6) , with C (x) being replaced by the sum of the coverages on the forward and reverse peak locations; and the associated discrete p-values were adjusted for multiple testing by application of the Tarone-modified distribution-free Benjamini-Yekutieli method, similar to a method recommended in Gilbert, 2005 . The Q value measures the statistical significance of the peak identifying the target region, defined as the estimated false discovery rate (FDR) among the rows whose Q value is no larger than a chosen FDR. The NLQ value is defined as the -log10(Q value).

Transcription Factor Binding Sites


GTTGTTGGCGATAC
GTTGTAACAGTTAC
GTTACGCTCGATAC
GTTACGCTCGATAC
GTGTAGGGCAATAC
GTGTAACTTTAAAC
GTGTAACTTACCTC
GTGCTTGCTGATAC
GTATTCTGCCGAAC
GTATTCGCTGATAC
GTATAGTATTTAAC
GTAGCCTGTGATAC
GTAACTGCTGTAAC
GTTGTTGGCGATAC
GTTGTAACAGTTAC
GTTACGCTCGATAC
GTTACGCTCGATAC
GTGTAGGGCAATAC
GTGTAACTTTAAAC
GTGTAACTTACCTC
GTGCTTGCTGATAC
GTATTCTGCCGAAC
GTATTCGCTGATAC
GTATAGTATTTAAC
GTAGCCTGTGATAC
GTAACTGCTGTAAC

Quantitative data format: NLQ, negative log Q score (range: 3.71 - 8186.60)

GTTGTTGGCGATAC 822.0713858
GTTGTAACAGTTAC 221.7559824
GTTACGCTCGATAC 893.1560559
GTTACGCTCGATAC 436.0281862
GTGTAGGGCAATAC 350.5286703
GTGTAACTTTAAAC 321.7870279
GTGTAACTTACCTC 103.1772378
GTGCTTGCTGATAC 479.500797
GTATTCTGCCGAAC 61.28176671
GTATTCGCTGATAC 2572.014529
GTATAGTATTTAAC 318.8299695
GTAGCCTGTGATAC 2974.315809
GTAACTGCTGTAAC 288.5646787
TAATCATCTTCTTAATTCTTAGTAATTCAGATGTGAGAATTACTTCTGTTTTGTTTTTATTTTTACTACCTTTTGGTCGTCCACGTCCACATATTTCTTTGGGTTTGATTTCTGTTGTTGGCGATACACTACTTTTTTCTACATCGCTCTTGATTACCTGTTCTATTTTAATCGGAAATGAGTGTCTTCGCTTCACACTTACTAATGATAATGTAAAAAAAGATAGCCCTAATATAGGTTT 822.0713858
CCGTTCATAACCATCTAGAATGCTTTGAATCAACAGATTGGCTTTTAATAAATCATTATTCAGTGACGAGGTTCTTTTTATCTTCCCTAGACCGGGAAATTCGCACTTGGTAAACACATTTTTGTTGTAACAGTTACATTGGGCTATCAATGCAGTTGGTAATGTTTTCCTTTGGCTTTCAGACTTCTGCCAACAGAAATCATCAGCTATGTCTGCCCCCATTTTTGATAGAAATACTAAATTTTCAAA 221.7559824
TGTTGTTTGTCAGCAGTCTAGTGTTAGTTGGAGACATCCTTTGCGATCGCTTTCCTTCAAAAGTTCTCAACTCAACCAAATACCTGTTACTGATCCAAATCACAGCCTGAGATATCAGTGTAAGTTACGCTCGATACAAAAATTAGCCCATTATACATAGAATAATGAACAAAGCAGCTATTATCACAGCATTTCTAGTGCTGCTCTCTGGAGTACCGACTGTAGCCCAAAGCAACTTGCCCAGCATGACAGTAG 893.1560559
CGGTTATATCCGAGCATTTAACATAGCCAAATTTCCTTGGCAGACTACTATGCGTAGGCGATCGCTTTCCTTCAAAAGTTCTGAACTCAAATAAATACCTGCTACTGATCCAAATCACAGCCTGAGACACAGATGTAAGTTACGCTCGATACAAAAACTTAGCCCATTATACATAGATTAATGAACAAGGCAGCGATTATCACAGTATTTCTAATGCTGCTCGCTGGAGTACCAGCTGTAGCACAAACCAGCTCGCCGAATATGA 436.0281862
CAATGCGTTGACAGATATCTTGCACTGCCTGAGATCCTACAGTTTGCCAAAATGGGTTACGCTTCCGCAGTTTAGCAATGTGAGCTTGAAGTTTTGCACAACTTAAGTGTTTGCCCCACATTCGGTAGTAGCGTTTGTGTAGGGCAATACAATGGTTGTAGATTACCCCACTCGCATTAATCATGCGCTTGAGGAATCTATTTCTTTTGTGCTGGTACAGTTTGAACTTCAAGGTTTTCATGTGGTTATAATATCATCATTGGTGTAGATAATCAACACTCA 350.5286703
TTTAATGCCAGAAAGTGGTTATACACAAACCTTGCACAACCAATACTCTTATTGATTAAGACCTCTTGATTGTGATTAGGAATGAGTGTAACTTTAAACGCTCTCTGCATTTTGGTTTTCTATGTACTTTTTGACTTGTGCTTCTGTATTATCTGAAACAGTACTTACAAAATAACTAGGATTCCAGAGATGACCTCCCCATAGCTTTTTCTTGAGTTGAGGAAACTTTAAAAACAATTTCCTAGCTGATATTCCCT 321.7870279
CACAACAAGGTTTTGAAATTAACGTTTATGTTTCCTAAGACTTGGAAGAATGTAAACAACACAAAAGCCTACAGCCAGATGAACCGTCACACATATTCGATGTATACGAGTTAACCCAAAAGTGTAACTTACCTCAAAAAATTGCTTCTAGCCCCAATGCAATGGTATTTGAACGACAGTCATGAGTAACCCTTTTTCCCATCCCGAAGATCCAGATTTGCATTTATCTATTCAGGAGAACC 103.1772378
CAACAAAATCATTGCAGAACACCAAGAATCACTACCTGCTGTACTAGCATATCTCCGCAAGATAGATCCGCTTGTTTTTGAAGAATTGCTTCTGTCTAGTTTTCAGCGTCAGGGTTACAAAATAAAACGTAACCAACGTTACACCGGGGATGGTGGGATAGATGGTCATGTTTGGCTGCATGGTCAAATGTATCTGGTGCAAGCAAAACGTTATAAAGATTCTATTAAAGCCGAACATTTAGAGGAATTTGCTCAAGTTA 695.3951156
TATTAGCTTGTTGTAACTCATTCTCAGTTGCTTTTACTATCTCAAGTATTTTATAAAGTAATGGTAATCCAGATCCAGATTTTGGTAATTGAGAAAGTATAGATGCAAAATCTTGTGCTTGCTGATACTCACTATCAATATCTTTTGCTTTTTCAAGAGCTTGCTCTAACAATTTAATACACTCACGTTCTTTTAAGTGAGTTGCCAGAGTCACTAAATCATCAAATTGGCAAGATTGATACTGAAGATTTTCTGCAATTTCAAAAGCTT 479.500797
GGAATCAATTGCAGCCCCTGATGACTTGGGGTTTCCGGCGAGGGAAGAAGATTCAGCTTACAACTGCACAACAGGTGCAGCAGTGTGATCGCTCCTACCCGGATTTTTGTATTCTGCCGAACTCGCCAGATTCGGATTGCCGTGATATTTCTTATCGTAGATTTAGGGTTAATCAACCAAATCCACATGGGTTCGATAGGGATAGGGATGGGATTGGTTGCGAACAGTAAAAAACTGCGTAAAAGGTTAA 61.28176671
TTCCTGTTTGGGGGCAATAGTACGCCATTTCTCTAGCAGTTCATGAATATCAGGCGACCTCTCATCTAAATCGTATTCTTCCTTGGGTGTAATATTGCGGCTAAATGTGTCATCTTCTAGATAAGCACGACGATTAGACAGTATTCGCTGATACAGTCGATAGTTTTCATTAATATAACTGCAAATTTCTTTAAGGGTTTTAGTATGAGCAGACGAATCAGTGTTTAAGCCATCTTCCCATTTACCTAATAAAGTTAATAAATACTTGT 2572.014529
AAACTAGAATAGTTACTATAAATCAGTAAAATGCTGTTTATCTGTATTAATTTAGACTAAAAACTTTATAATACTTTAAGAATATTTGACTGGCTTACAAAATGGTGTGTTTTTTCCTTACTGTTATGTATAGTATTTAACCGAATTAAGAGCTATTTTCCTTGCCATAGCAATCATCAGCTAAAGTAAAAAGATAGTAAAAATTTCTGGTTCTTCCGGTAGTTTTATGGTGTTTATTAGAATTGACTTGAT 318.8299695
CAATGTTTCTGCGAGATGGGCGATTTTATAAATATCTGCCTCAACTTTTTGCACGGGTTAAAATGGCATTGAATATGAATGTCAGTAGACCGAAAAGTTTGGCGATCGCTAAAAAATAGTAGCCTGTGATACCGTTTTTGGAGAGAAGTGTAAAAGCGGATTGCTTGGCATAAGTGATAAGTTTAGCTTATTAAAAACCCCAAAAACCAAGCAAAATTAGCCGGACGGTAAATAAACTTCG 2974.315809
CTAATCCTACTAACGGAGCAAATTTAGGTACACAAAAAACAGCAACTCTAAATATTGTTGATAATGATGCTGTAGCAGGAATTTTTCAGTTTAACAACGTCAGCTATGCGATTAATGAAAATGGTACACTTGTAACTGCTGTAACTTTAAACCGCACAGGGGAGAGTGATGGTGCAGTTAGTGTCACAGTTAACTTAAGTAATGGTAGTGCGATCGCCTCTGTTGACTACGATAATACCCCAATCACTGTCAACTTTGCCAATGGC 288.5646787
CACTTGGTTGTGTTGATTTACCTAGTTTTGCGCGAACTTGACCCCGCAGAGTATGGTTTAATTTTTCCCAAACTCCTTTGCGCTGCCACTTACGGTAATAGCTGTATACCGTTGAACTTGGGGGGAAATCGCCTGGAAGCATATTCCATTGACATCCAGTCTTCAAATGATAATAGATGGCATTGCATATTTCACGCATATCTGTTGTGCGTGGATGTC 23.17503614
AATGGTGGTGCAGCCGGGGTAAGACTCTCGCAGCAATTAGGGATAAAAGTTTCTCGCAATACACTATTAAATTTAGTCCGTTCTATACCACTACCACCAATCGTAACGCCACATACTCTTGGGGTAGACGATTTCTGCTTTCGTAAATGTAAAACTTACGGAACAGCACTAATTGACCTGGAACGCAGACGACCAGTTGCTCTACTAAAAGATGCTAAGGCTG 35.95278271
TATCTTTAGAATGTCGGCGACAGCAAATAATATTAGTCCCTCAAGAAGCCCATTTTTGGAGTCGTTCAATTTTTGATAACTTCCGCTTTAGTTATCCTGATGTTACTTTTGAACAAATTGTCAAAGCTTGTCAAATTGCTGGTGCAGATGAATTTATTAATGAATTACCGGATGGCTACCAAACTGTATTAGGGGAATTTGGGGTAAATATTTCCGGTG 69.38554533
AGGTAAATCTAGCTCCAAAAGACGGTACATCCTTAACAATACCACCGAGTGAACCAATATCATCTGTTGCTTGGTTAACTGGATTATTAGGAGTAGTCCGGTAAGAGAATGAACCTAAAAATCGAGAAGATGTCACAATCGCTACTTGACCAGCATTATTAATACTTTGAGCGAAGCTAAAACTACCAAGACCAACAAGACCAAGTTTATCTGTTGCTGCATTGACTGGACT 44.41651689
CTCATCAAGCCAACGACCTGACCTTGATTGTTCATATTAGCTGCACGGATATCTGTTCCCTCTGGGAGCAACTTACCAAGGTCAAGGACAGAGTAAACTGTTGCTGCGCTACTGGGTTGACAAATAGCTATTCCTAAACCAGTCAATACAAGCGCTGTTAGAGGGTGTTTGAAAAGTAGG 8.859329722
AAGCCGTTGGAACGGCTAATTAACTGCACTTTACTAGAAGCAATCTGCCCTAACCGTTTCCAGTTAGCGTCGCTGACAGATACTTGTTTTTCGGTGTCTCCTGACACATAGCCAATCCCTTTAGGGGAAGTAACTAAATCCCCCTTGCGTAAGCCATGACGAGTTGTAGAACCGCCATATTTACGACGTACACCACTTTTTGCAGGAACCATCAAGTGAAGTTGGCGGCGAGAGTAAGGAGGACGACGAATTAGTTT 172.8200873
CGTTTTAAAAAATTAACCTTACGATCAATCCTTCTACCTCTGCGACCACGCCGCATTAATCGTCTTGCGTCCATGCGATCACGCACTGTTTGGAATGGCAAAATCAAGTGAGCCGTGTACAGTGTGAACTTGGCAGACTGTACACCGATCCCAGAAAACTTTTTACCTGGATCAATCCCAATAACAATATCTTGGGTTGCTCGTCCAGATGGTTCACCAGTAAGTT 60.09332053
ATTAAGAAACGGCTACGCCGAAGCGAGAGTGATGCGTTCTCCCCCACAACGTAGAAAAACAAAGGCGATCGCTCTGGCTGAGAAACTTAGCGATCGCCCTTTACTGAAATCAGGTCTATATTATGTCATACGCAACAATAAACAAGCAAGCCCAAAGTCCGTTAACCGAGCAGGACAAACGAATCATCGCAAACATTATCCAACGCCATCCAGACGAAATCAAAACCATCTGGATAGACTGCGGAATCACGGTCTGGGTGCAACTCCAAGACGGTGGAAGACTACCCTTCGACAAAAATTGGTTCGCGCAAAGAGTGGCCCAAGAAAAGACTTCTCTAGATGCTCAAGAAAGCCTCCACAGGCACAA 24.78515219
CCAATCGTGATTGCTTAGTTCAAGCTCTAGAGGATTTGAAGCTTCAGCCCCATGTTTTTGAGCAACCGCAGCCGTTAGCTGGCTATTATATGGATTCGCAGGGGTACAGTGCAGAGATTATTATCTTCGGCAGGACAATCAAAGCCCGTGCAGACATTGGTTTTCGCTGGAATCAAAGCAGTGGTGTGTACGAGGTTATACACGATGAGTATGAAACATCTCCGCGACTTGGTGAAGATTTCTTCAGCCACAAGTTAATGCAGACTTACGGGCGGCGGATGGTTTTAGCCAAAACTGAAGAATT 52.18611144
AACCAAAGGTCAAGTGCAAACCCTACGGTTGACTTTTGCAGGACATCAAGAAGTTAAACAATACGCCAGGAGATAATCAATGGAACGTTCTATACTGATACATTTCGATAACAAAACGGGTTCAGTGCGAGTAGAAGCTCTTGGGTTTGAGGGGTTGTCGTGTTTGGAAGCAACCCAGCC 54.87954831
CTTATGTGTGCTTCTGAACGCCATAGTTACGGCAATTGCACACCGAGACTGTAACGGTGTAGCTGAAGTATTTGACGGCGATGCCGAAGTTTTTGAAGTGAGAGTTCAAATAGTTAAGACTCACTCTGAACTTGCACCACTACCGTATAAGGAATTACACCAATGAACCTAAATAATATATTCTCCACGCTGGATTCACAAATACCTATTGTGGCATTAGAAGTTCTCTCGCCAGAAGAAGCCACAATCATCCAGTGGTTA 232.5099659
ATCCTCAATTTACCACTACTCAAGCTTGATATTGCTTCGATGCTTGGCTCTTTGGTAGGGGAGTCTGAGGGCAATGTCCGCCGCGCACTCAAGACAGCCGAAGCGATCGCACCCTGCGTTTTGTGGTTAGACGAGTTGGAAAAAGCCTTATCCGGGCAGGGTGATACAAGCGGTGTTTCCCAAAGAATACTCGGTACTGTCCTCACTTTCATGAGCGAATGTACTTCGGGGGTGTTTGTTGTCGCAACTTGCAATG 13.11723034
ATGAAATAGAGCGCCAACTGCATTAAGCACTCAACGACTCCGGTTGCGTATCGGCTATTTCTGTAACTCGGTATAACCAATTTTGAGTTTGGGAAGCCGCCGATTGCCTTTGTTCGGCATCAGACCAAATTTTGTCCCAATCGAACAACCAATTTGTCATTAAACGTACCAAGTCAACCAACTCACAAGCTCTGTCAACGTCCTGAGCCTCCACCATGTCAGGGAC 18.60150378
AGCCGTAAAGAGTTTGAAGCCGAACAAATTAGATTTACACAACCGTTAACAGGAGAATTACAAAATGTCTAATAAGGATACATTCACCCGTCAAGAATTATTTGAACTGTTAAGCGCCAGTTATCCCCCAGAGAAAATCATTGCAGGGTTACAAGAGTTAGCCAAAACAGACCCAAGCATTGATCCA 38.10511207
CCGATGGATTGTTGAGCTAATTTCACTACCAGTTGTGTTTGTCTCACACATCGTTGCTCAATTCGTAACCCCAGGTACACCAGGATATAGATTAGTCGGTGCAATTGGTTTTTGGATTGGCACACTGCTTTCTACTGATGGTATTTGGCAAGTTTTATTTCAAGGCAAACCCAT 26.46843183
CCATTTGGATTCATTAAAAATAAATTAGTAGTACCTTGAGCGTAAATCAATCCTTGAATATCAGAAGGTGTACCACCAGTTACTCGTGCAAAGATATTAGTCAGATTGGGGTCATTTAAAAAAATTGCTGCACCACCGTTAGGAATGTTGAAACTACCAAAACTATGGAATAG 34.16215413
TAGGGATACGAACGACATCAAATCCTTCGACTAAAAGTTTGAGATTGCCGTAACCGTTATCGAACCCTGCGGGAAATATTTTTTGTAATGCGTGAACACTTGTCATTAATGTTAAATATGATACAAAAATTGATGGCGATAGAAATAACCTAAAAGCCACCTAAATATATCCTATATGGGTAACAAAATGAACCAAAAGATTTATAAATGATTTTGGGATAC 204.9139208
TTTAAGTTACTGAAATAGTATTGATATACTGAGGTGTTTCAATGTCACAAGTAGTAATAGCTGTTTTAGCAAATGCTGGCGGGGTAGGAAAAAGTACTTTATCAGTACACTTAGCTTATGAAGTAAGTCGGCGTAAGTTAAAAG 15.03267916
CAGACAGATTACAAAAATATTCTTTACCCCATGATCTAGTTATTGTAGACTGTCCTGCTACGTTGGGAATGCTTAACGTCAATGCTTTAGCTGCTGCAACCCATATTTTAGTACCAATACAACTTGAGATGAAAGCAATTTCAGGTTCAGCAGAATTGGTAGAGTGGTGCATGAATACATCTGAGGAATTACAATTAACACCTAAGCCTCAGATTCTAGGTTTTGTACCTAGTATGTACGATGAGACAGTAGCTATGCACAGGCAGTACCATTCACAACTAC 25.16611856
CGTTTTTAGGATTTGAAGACTTAAACCCATTGGATAAGGCAGAGGCTGTAATTCAAGAGGTTATTAAGGCAACTGCATTAAGTTCTGAGGAAATCACTACAGCACTAGCAACTGTACTCAAACGTATTGAACGCGATAGTAACATCAAAGAATTGGCAAAATTGACAAGTTTAGATGTAGATGAGCAAAAACAAGGATTAGAATCACTGGGGATTATAGGTGAAGAACAAAATTTGTTTT 22.16690726
TGTCTCGCGGCTTGTCATCTGTTCACATTGCTTACAGACGAAGTTGAACGTGCGGGTGTGTATCGTGCGTTTGTGCGCTCTGACAGTGTATTCTCTGACATCGATTTCTTTGCTTGCCATTGCTTATTTCAATTGTTAACTTTTCGGATCTTAGCCTTTGCCAACAGGATTTCAATAGTCTGGGT 30.00635743
TGATTGAAGATGCCCGTAGAGATTGGTGTGCTTTGGGCGGTAGTGAAAAGGACATAATTGACCTAAGTAACATTAAACTTG 5.105970867
CACCCAGATACCGGGAGTGTAACTAACTACCTGAAGGTCGAGCAAGTTCAACGAAATAACATCAGAACTGCTGTCGAGATGCTTGAGTTTGCTGCTAAGTACCAAGGACAACTCATGGTAAATCAGAAATCCAGTAACGCGGCTGTATCCATTCCTACGCACAGTATATTCGACTGTGATGGTGGTGTTGTGCCACGGGTTTTACTCGTCCGTCCACAGAAAGAAACTCGTGTACCAGTCGAACAATTGGAATCATCCACTTGGCAGCAGGTTTCGGCTGAGGAGTTTGTTACGGCGTGGTCGAGAGAAGTTGATGAATTACCCAGATTCACTAC 58.15118767
CCACAGCTTTTCCACAGCTACCTCGGCTATTTTCCACAGACAGTCAACAAGGCTACAGGCTTTTGCCGTGTCGCTGGGGATTTTATCTTCTAGGCTTTAAGTCTTGTTAATTACTGAGTTTTTATTAAGTAACGTAACAGAAATGTATCTTCAACCCTGATGCTGCCGTAATACTTAATTCTTTTTGTACTTATGCTTGACATTTCGCAAC 9.828876297
AAAGCTAGTTGTCAGGTTAATTCTCTTGTCTCCAGTCCGCATCCTATGAACGCAACAGTTAGCATTTTCACTCAAATCCCTGCCTCCTTGCACGATTGTATGCAGGATTACTTACAGCAACACCCAGATTGGGATGAGAAACGTTTACTGGCTGCGGCTGTCTCACTGTTTCTGCTGCAAAACGGTGATGGAGATCGCCATGTTGGGCAAGTTTATC 38.75677149
CCAAATTGATACCTATGCAGAAGCTAGAAAGCCTATACTGTAAGGTATCTGCAATTTTTATGATTGTGATTCGCTCCTTATTAAAACTGCTATGACTGATACACCCAGCAACCGACTAGACCGGATTGAGGCAATTTTAGATAGGGTAGCATCTCAACAACAACTAAATACT 25.17581965
ACTTTGCTGAAGATTTTCTGCAACATCTGTGGAATAACTATTACCGCAAGTATTTGGTGTCTGAGAAGTTGGCAGGCAATACAGTTGGCAGCAAAATCTGTCGTAATCCTGTCATCTGGGACAATCTCTACGTTTCAGCGCAAAGCTACCTGAATCAGCAGTTACTGAAGATGGTTAGCTCCAGCACTAAGCGCGAAGAATTGAAGCTGGTGAATTGACGATAATTATTGGGAAA 24.72096952
ACTTCCTCGTCAGTACAGTAATTGTCGAGAGCGATTGCTTGGTGTAAAGTGATGCCATCGCCAAGTTGTATATGTGCAAATGCAGTTTGAATCAGATCGATAACTGCTTTTGAAGATAAGAATGCT 21.18549619
ATAGCTTGATTTTTTCTTGTAATGGTTTGATGCGGTCACTTTTAGATTTGACCTCAACAGTGAGATCAGGTACTAACTCCACAAAGTCACGCTGAGTTTTTTTTAGTCGTTCAGCGCGAACAAAAGACACATCAGGAGCGCGTAAGTTTCTTTTTTCAGGGTTGCCTGTGTTGTCATCATTTTCCAGACTAGGCAAGATAAAGCCAGCACTAGAACCAGTCACTCT 61.27087461
GTTAGTTAACTAACTTTCTGCTCTGCAAACAGTGACAACTGCCCAGGAGAAACATTACCTCTGCCAAAACGGTGGTATGACAGGTGACAGCCACTACACAGACAAACTAAGTTTTCTAAGCGATTGTCAGACGGATCTC 14.93979402
GAAAGTCCCACTGGCAATATCTCTAGGATTACCAGTATTTGGACTGAAAACCAAACCGTTGCTTGTAAATAAATTGATTTGTAAATCAGGGTAAGTTCCTGTATCAGGTGCAGCAACTTGGTCTTCTAATTCTGCTTGTGTAGGATTCGGGGCAAGCACTCCTCGTAGCCATTCTGAAGGTTTTAATGTCACTCCTTCTGGTAAACGTA 32.20056815
AACAGCACTGTGTTGTTGTGAAGCGAAGTCTTTGAGAGGGAAGTCTCTGTTATGATCTGCCGGCTGTGAGCAGATCGATATGTCCAAATTGCTCACATCGATCTGTTGATTTTGACAAATGCGATTCCACAAGGCTTGTACGTTCTCGACGTTAACCGTGAACCAGTTCGCTTGATACCAAGTCTTCTCACTGTGACGACAGACATCAATCAACTTAAAATTATCTCTCAAGTTAGCGA 47.00530112
CTATGTCAGAAAGTTCTAAGAAACAATCTAGCTTTGATCTTAAAGGCGCTCAATTCGGTGGCGGTCTAGTCAACGCCGATACAGTAAATGCAAACCAAATCGGCGGTAACATCACCAACTACAACCCAGAGCAAAAGCAGAATCTCGCCCAAGCCGCAGCAGACATTCAGCAGCTTCTCAACCAACTGAGCCAAACCTACCCCACGACTACAACA 45.63946678
GAAAATCAGAGACTAGTACTAGCTGATGTTAAGCATGAAGTTAATGGGCGACTGAAACAATCTCTACATAACACTGTGCTAATTAATCTGGGAAAAGAGTTACAACTGCAACAAGTAAAAAGATATTGGGATGCGGAGATAAAAATTGGGACTAAACCACCTGAACCCGTTTCAGAGAATACAACTATCTTATCAGTTTTTGATTCTGAAGAAATAGCCG 115.3563431
AAAGATTAGCTTTCCTCAAGAAGCTGTATCAGCAGAAGCATGGTTCTCGACGACTTTAAATATTACTCTTTTTGCATCTATCACTGGACTCGTTTTTGGGTTAGTTAGTGGATTAATTAGCGGTAGAGTTTGTATTGAACACTTTACTCTCCGCCTCATCCTTTACTGTAACGG 31.04220086
TTTATCATCAAGATTGCCACCTAATAATTCATCACCGTAGCGAGTTCCAGTAATATCAAAACGTTCGATATTACTGTAGCGAACATGACTTGGCCCCCAAGTAGAAATTTCACCACTTCCGGTGGCTAAGTCTAAAGAGTTATAGCGAACGCCGATATCAACGTAAGAAGACAGAGCAAGGGAACCGTAAAGCCCGTGATTGCTTGTTTCATCACGATTAACT 23.17503614
ATCGCCATAGCGAGTTCCGATAATGTCGAACCTTTCAAAATTGCTGTAGCGGATGCGACTTGGCCCCCAAGTGTAAATTTCACCGCTACCTGTAGCTGGGTCAAAGGAGTTATAGCGAACGCCAAGATCAACATAGCTAGATAAAGCATGACCTCGACCAATACCTATATTGGGTGAAGAAGCATCAATGAGAATTT 27.69750178
CTGTCTTAAATTTTTACCATCAGCGTTAGCAATGAAAATTGTGTAGGTAAATTTGTTGTCGTTACGTTTACTACGTAACCACGCGATAGTGCTACCATCCTCTGAAATAGTCGCTAAGTAGTCACCATCGCCATAGCCAATGTTTATACTTAACTTGGTCAGTTGTCGAACTTGAGTTCCGTTAGTATTAGCAAT 44.02721634
ACTAATAAAATCGTTACCCTCGCCACCATCAAATTCATTATTGTCCTCATCACCAATTAAGGTATCGTCATATCGAGAACCAACAATTTGTTCGATAGATTGAAAGACATCATCGGCTGCATCTCCCGTTGATACCTGAGTTACTAAGTTAATAGAGATAGCACTGGTAGCACTTGTATAACTAGCAGTATCAAAGCCTGCACCACCATTAAGGATGTCTGCACCTGCACCACCATCTAACCAATCATCACCATCATCGCCATAAACGAAATCATCCCTATCACCACCATAAAGACG 40.70620084
CAAAGGCTTGAGATGGTTGAGTAAGAACAGGTATTTCTAATCCTGGAAGGCTGAGAAGGTTATCAAGATAACTTTTCTCCGAACTACCATCTGCCACCTGATCATTCCAAGCAGAAGCAGAACCATTGGTATTTGGGTTAACATTTTCAAGAGAAAAATTATTAGCACGAATATCTTGATTGCCAAAGTTAAAACTGCCTAAATTTATA 137.3986377
TGCATGTTTTAGCTGTGTACCTAATAGCGGCAAACTACTGCCAAAAACTTGAGCGCTAACTGCCGCTTGAAGCTGAGATAATAGAGTATCTAGTCCGGTAGCTACCTCGTTAAGATTATTCCCACTGTAAGAACCGCTACGCTCAATTTCTACAGTTTGCCCATCAAGCCCGATCAATGCTTGATCATTTTCTAGTTTTAATTTTTGTAATTCTGTTTGGGTGAGTTGTTGTC 25.69662326
TTTCTGGTCTAGATATGCGCGAGTGTGATTTACGCACAGTAAACTTACACGCAGTCAACTTTACACAAACAACTTTTCGAGATTGTGTTTTTGCTGCTACCTTTGGTGGAATAACTAGCGTCGCTTTTAGTCGAGATGGATGTCAACTAGCTACCAGTGATACCAGTGGCGTAATTAATATTTGGGACGTTAACAATGGTAAACAGTTAT 68.71474181
GAATGCTTCAATACACTACATGGACACACTAGCATTGTCACATCAGTAGCTTTTAGCCCTGAAGGAAAACTGCTGGCTAGTAGCAGCTATGACCATAGCGTGAAGGTTTGGGATTTAGATACAGGCGAATGTCTACAAACTTTCTTAGGACATGATGCTTGCGTTTGGAGTGTAGTTTTTCATCCCGTAG 25.432685
ACGGCTTACAACAGGCGCAACTCATCTTTGAAATAGCGGTCATTCTTTTTCGGTTTGTCTTGCCAATGCTCCCAAGCCACCCAAACCTCATCACCCAAATCCTCAATAACTTCACCCCTGGCGTTATACAAAGCACAGTAAATGTCGGCATGGGCAACGATAGAACCAAGGCCAATCACTTCAGAAGAACCAGACTCGGCAGGGGTTTCTTCAAGAACCATTGGTTCCGCAATG 36.49023454
CGATTAACTGTACTTAACTAAACATTGCTGGACATTTCATGCTAGTATAAATGTATTAATACAAAAGTAGTAATGACTTACTATGTCCCAGCCAGAAAGCCACAATCAGTGTTTAGAGTAACACTTAAAACACTGCGGCGAGGGGAAGAATCAGGAAAGATTAAGA 22.58898178
CTCAACTTCCGAAGAAAAAAGTTACTTGCCATACTGGAACGAGTTCTCGCAGGCGATGTCAGAATGGTTGTGGTCTGCCATAAAGACAGATTATGCAGATTTGGA 6.20348106
GAAAATGATTTTACAAGAGCGCTATGGGCGATCGCAAAACTCTCAGGACGTATTTCTGCATCGGTCTTGCAAATGTATAACCAAGTTACGGGAGTTGACCCTGCCATTGCATCCATCAGTCTTGGTGCAGTACGCAAATTTCAGATTAAGCCAATCTCTGGC 21.5333687
CCAGTATTCGGGTGTTACTTCCTGACCTGGAGAATCAATGCCTTCTTTCAAAAGTGCTTCTAAATTCGCTTGCGCTTTGTGTTTTTGGTCTTGTTTAACTAAAGCTAGGAAGTATTCGCCAATACTGTTATAAGCACCTGTCACCACTTGTGCCTCCACATAAACGCGTACTTCATCAGGTATATCAATATTGATATTCATTGGCTTTCAAGCATGACTACTCTAATTATT 136.4284302
TTTAGAAATCAAGAAAAGAAATTAGTATTTTATGGTTTTACGGTTAGAGATTGGTTTTTAAATTGCCAAGAACTAACTCTTAGTGAGCTAAATTATGCTATTGAGCTACTTGAGTGTCCAGAACAAACATTTGAATTAAGTATAGAATCTTTAGTCTACCGTCTACGAAATGCTCAAGAATGGACAGACATCACCAAAATTATGTCTGAGCGTCCTGAAAGTATAAAAAAACTTATTTGG 141.6392177
TTGTTATTTATCAGAATTGTATATGGACTAGGTGACTTAGTGTTTTTTCGTCTTGTTCATCATCAGAAAGGCGAATACGGAACATTTGAAATGAAACTTCCCAGTATTATTACAACATGGGGAGAGCGAATTTTTGGGCGGACTGTGATTCAAGCAACAATTAATCATGATGTCCATCATCAAAATCCTCGAATTGCCGCACATAATCTGGCAAA 64.2383596
AACATATCTGATCATGAATTAGCTCGTCGTCGTGCTAGTTGGCAACCTCCTGTACCACGTTACACTAAAGGCGTGTTGGCGAAATATGCCAAATTGGTATCTTCTAGCAGTATCGGTGCTGTGACAGATTTGGATTTGTTCAATTGATATATAGGAGACATCCCCAGAAATTAGGTTTTCACCAAGATAAGATAAAGGTTTCAG 44.5607725
AAATGCTGCGGTCAATGGTGTCTTACTCACTAAATTATTAAGCAGATACTCGCTGAAGTGGCGTAATAGAGTTTCTGGAGCAAAAAATTCTAATTTATCAGTTTGTGTGCTGTTACCTGCTACACAAATCAATGGCATATATTCAATATATTTTTGAATATTGGCTTCGTATTGCTCGGAAATTATTTCCCAACCAGCATATATTTCA 227.5625881
TTTTGGCTGCTTTGCGAGGTTACAGTTGGTGAAAAGACAAACGAGCGATCGCCCTTGGCTTCACTCCGCCTAACTGTGCGGCTTGCTGGTGTCGCACACTTTACTCTCTAGTCCTGAAACATTGTAGTTTGCAATACCCCTTCAATCGGGTACTGGCATTGATACATGAGCAGAGTATTTCTCTGGTATTCTCTGGTATTCACCTACTCCCGCAAGGAGTAGGTGAAGTTGACTCTGCTCTAGTTGCCCTGGAGGGTAC 233.9960987
CTTATGCAGATGAGCATATTCTAAAATTTTCTCCATGAGGATAGAACCAATCCCTTGACGGATAAGATCGCTCCTAACATAAGTAGCAGTTACATGACCATCTTCTGTAATTCCTGCAAACCCTAAAATTCCCCTTTCATCTGTAGCTATAAATGTAGTGGCGTTCAAAATGAACTTTTGAAAATTATCTGTATCTGATGCAAAAGATGCCCACATTTGCGTTTGCTCTGGAGAGTAGTGCTGCGGTGCAACTGCCAACACTGTTGTTTGATAAAGTTCTGCCAATGCT 242.9572076
TTGCTGTACAGAGGCAAATACTGACCTTTCTTTTTCTTTTTGGACAGCAATGCGATTGAGTTTGTCCTGAAGTGTTGAAAAATACAAGTAGTCTAATACTTTCTCACACAAGGTTGGGTCTTCGCCAAGTTGTACTTTTTGGCACAGGAGAGTTT 15.67193721
CAATCTTCGCTTACTCATAACTTTTTTCGCCAACTTTTATAATTATTAGCAAACCCTACTAAATAAGTGGGGTTACTGTTGTTTTACGTGTGGAAAATGTAGCGTAAATTACTATGCAAAAAGAGTCATAAATAGCTCCTAAAAAGGTGTTATCCACCACCTTTTTTGGACTAAAAATGCAGGTTTGTTATCTTTTTCTGACATTCTTGT 148.9485399
GTGATTATAGTGACTGTATTACTGGCAAAATCTTGTAGATAGCGGGAGAACTGCCCCAGAAGTGCTGTAGGATCGACTAAAATAGTCGTACTCACTACGTTTATCTACCCATTTTCGCATTTGAGCGATTAGGCTTCAAAAGTGGCGTAAAATCGGGGAAAAACAGCAATAATTTGCGTGATATTTGTTTCTCCCTCAGCCAG 26.66722518
CTCAAAGCGCACCCCAATTTACCCTACCCGTTGAGGAAAAATCTAACTGGTCAAGAGTTGAAAATTACCTTACCCAAAAACGGGGAATCCCCTCTGATTGCGTACAGATGCTACATAACCAAGGGCTAATTTATGCCGACTCAAAAGCAAATGCCGTGTTTGTCATGCGGGATCTCGACGGGAATACCAAAGGCGCATTTTTA 68.62325936
GCTATTTCACTGTTTGTACTGGAATATCACGTTAAAGGAATACCAGACAACAGAACACTTTACATGGCAGTAGACGACACCTCAAGTTTACCTTTTGAACGATTACGTCATGTTCCTCATGTACAAGTAGCTTTTATTCAACCAAATATGGCACGGACTGTTAAAGAACTACTGCCAAAATCGAAGTTACTCAAGTGTGAAACTCTTGATTGGAATACGCAGTTAGTCAATTCCTCTCGTCAATTACAACAAC 54.24919925
CAAACTTAACAATAAGCTGATAGCTGTCGAGTCTCCATTACAAGAAAGACTCAAACTTTTGACAAATCTTGCCAGTCAATGCCAGCAACATAACATTAACTGTTACCTATGGACGCTGGAAGATGACGCGCTACACCAGTTAGAGACTAATAAGGAGGGATTAACTTTACATCCAATTAACCAGTATCAGGCGATCGCCAAAAACCGACGCG 90.93236041
ATCTACTGCAATTTTGTATTTTCAAAAACAGGTATTTAGGAAAGTTTTGAGTATGTACTATTTATTAGCAAAAACAACTGGCGGACAACTAGCAACTGAAGCAAACAATAATGCGATTGCTGTTGCCCAAGGATTTAACGAATTGTGGGAGCGAACTATAAGCGGTGAAGTTTACAGAAATATGTGTACTATTGGCGCTTTATTCGCTGTAGCTACGTTAACATTCTTCATGATTGAATGGACAAAGAAAATGGTGGCGGGGGACGAGCAACGGGCATATACAGATTTTATTTGGCCTTTA 79.70949121
GAAATGGTCTGATTTCTTCTAGAGAGTATCCATAAGCACCATCAATTAACTGATTTGCAATCAACGATATTCCTTTCAACGAATTTGACACATCGTGCAGTAAAGCTAATATATCCAACTGTTTGCTTAAAACTTCCTATTGCATAACTATACTACCAGCGAAAGATTGTTATTTTTGCTACTTTTATGACTGAATGCTAATTCAGTCTAGAAATTTTAGAATCTTTTTATTTTACCCATCAAAAAAATTCATCCAATCCAGTTTTAATCCTAAAAA 217.2973062
ATTGCCGAGCAGTTATTCATTACTCAAAGCACTGTCAAAGGGCATATAAACAAGATTTTTGCTAAGTTAGATGTTGCCGATCGCGTCAATGCTCTGATTGTAGCCAGTAAACTTGGCTACATCGAACAGGATTACCTCAAGGTCAGTTAATTACTCATCTATTTCAATCAAGCAATAGCCTTTGATTTTACTCTGCTGTGTTTGGCAAGCTTTAATTAATGCTTGATTTTTCAAGATTATTTCATAC 90.1997547
GAATTCTCTTAATTACGCTGCTTTTAACAAAAATTTATTTAATGTAAATATAATTTTTTGCCACGATGCCAAAAAAAGCCAAGCAAAAGTTGTCTTTACTTCTACTTTTATGGCTATAATGAGCATAGTAAAGCAGCGTGGTGTCTTACTGACAAGTTAGTGGTTTTAAGTCGCCAAACTATCGCCCACTTTCTCAGTCATCAGGAGATCAACCCCGCTCTGAAAATATTAAATTGTCCGAATTTCAGGCAAAGCGGCACTCCTTA 59.21000627
TTCACAGCTAAGACGAACCCATATTCATTTGACTGATCGAGTTTACCAGTGATTGCTGCTACCCACTGCGGGTAATTAGTAGCAGCATCGGTCAGCTTCTTCCATACCTTCGGTT 8.291402172
TTTGAATTGCTACTTTATAATATTGAATAGCTTCCTGATATTTTCCTCGTTTTTGATATAGTTTACCTAATTGCTGTTGCCATTGATACGCTATATCCCATGCTTGAATTGATTGAGCTATACTTAGCGCTTCACTTAAATAAGTATAAGCTTGTTCTGGCTGTAGCTCTCCTAATATACCATTGCTGTACGATATTAATCGCTTGTTATTTATACTATTAGCTTTTCGTAAACTGGCTAACGCATACTTTCT 159.5956428
GCATTAGTAAAAGTAGTAGTTGCTATTTGTCCACCTTGAATTGTTACATCTTTTACGTCAAGTTGAATAGACTCGCCAGGAGTAAAGCCAAAATTGCTTGTATATATTGCACTTAAAGCTAGATTAGATGATCCTTGAATATTTAGGGATTGAGCATCAATTTTAACAGCACCAGTTTTAAAGCCATGATTTTGTGTAAATACTAATGAACCATCTAAAATGTTAATATTTTTACCTTGAATATCAATAGT 39.13925763
AATAGAAGCGTTATTAAAATATGCAGTGTCTCCATAATTAATTGAAAAATCTTTAAAGCTATGAAATAAATTCCCTCCTAATTGAGTGCCGCCCTCAATAAATATTGTATTACCTTGGACATTTACGGCACTATTATTTTGTGAGCTATTATCAGCATTTATTTGAGCTTGACACAATGGAATGAAAGTTGTGAAAATTGTAATTAAAAAAGATATTTCTGT 178.9397151
TGGCTTCCTTTTCTTTCAACTCAGCAGGTAATGTGTCGAGAGACTGTAAATTTGCTGCAAATTCTAGTAGTATCCCATTTGGATCAAAAAAGTAAAAAGAAGACATGGGTACATCTGTATGATGCAATACTGGCGTTGTTTGTACTCCTTTTGATACAAGCTTTTCTTTGTACTCTTCTAATTTTTCTAATGGCACATGAAAGGCTACATGATTCATAGAACCATGAGCAGTTATGATATCGCCTGTC 133.6818873
TTATCGAAGTAAAATCATCTCTTGCTTAAAAGTACTGTTAAATATTACTGTGCATATAAGACAGATGTGTAAAGTATCTTTCAGATAGTAAGTTGTTCGTTTTAGGAAGTAATGTCTTAAGTAGAAAAAGAAAATCAATGAACAGTTTTCTTCAATTGCTGTAGAGTATGTATGCACAAGAAGTTAACAGTCTTGTCAA 24.79053699
CTAAAAAAAGGATGGGGATATATGAAAACGTTTCTACTATGATGATACAAGTAAATAGCTGTTCAACCACCTTCAGTCTGGAGGTGGTAGCATGAACCAACCTCCCAGCAAGCGCAAAAG 11.06593249
CAAGTAGGTGAAATTTGCGTTCTGCATCCTAAAGACAACCCCGACTTGAGGGGCAAAAGTGGCTACTGGGGTGTAGTAACTCATGTAGGTGAGTATAGTTGCACAGTCAAGTGCTGGGATGGCGACTATACAGCCAAAGTTGAGCATTTGAAATCTCTGGAATTACTAGA 11.78818949
AGACTTCATCTTCTATATTCCCGCAGCAAAACTGTATATTAGCTGCAATACCTCAAGGGGAGAAGATAGGCAAAGTGGACTGCTCTAAACCATCAAATCAATCAAAAGAACGGAACGAATAAAAACGCCATAGAGGTCTAGGGGAAGGTCGAATCCCTGGTAAGTATGACGAGATACAGAATTCCTCTATAGCGGGTAGGACAGAGCGTAACTGCTCTGAGGTGTTCAGAAAACGCGATCTGGGAGTAAAGCATGGTTAGACACA 110.0492171
CGATTCGTCAAGTAACTCAGCTAAACGCTGGTAAAAACACTGCTGGTATTGATGGTAAAACTGCCTTAACTCATGAGGAGAGATTTAACCTCGAAGTGCTGTTAAGACAGCAAGATTGGTATCACAACAAACTCAGGATGATACCAATTCCCAAGAAAGATGGTTCCATCAGATACCTCAAGATTCCCACTATTGCGGATAGAGCTTGGGTGCGATTCGTTGTTTAGTGAATCACGGTATAAAGTCCGTACATAACTAAGCCAACTGTAATTAA 116.826537
CATAACCAGGCAATAGCTGCAAGAATCTTGGGTTTCTGATGGTTAAAGTGGCTACACCTAAAGGAACAAGTAATCATCTGAGGGAACGAATAAAAACGATTTGAGAGTCATGGATACGTCGATTTTCCATGTAGGTGAGGTGAGACACGGAAATCTCTCAAGCTGGGTAGGATGCAGCGTAATTGCTGCAAGGTATTCAGAAAAACACTTAGCGGAGAAGAGCATGATTAGGCACAGTGTAAAAGCTAGTGAATCTTGGAAGAATCTACCGTG 30.5866084
CAAAAAGAATATCTAAGCGAGTAATCGAGCTTGATATCGAAAAGTGCTTCGACAGGATAAACCACTCAACCATCATGGAAAATTTGATAGCCCCAAAAGGTATCAAACTTGGTATCTACCGATGCTTAAAAGCAGGGATAAATCCAGAATTTCCAGAACAGGGAACGCCCCAAGGTGGAGTGGTAAGTCCATTATTAGCCAACATCGCTCTCAATGG 44.62172507
CACCAATAGTTAGAGGATGGAGAAATTACCATAGGTTCTGTGATATGTCAGGGTCAAGGTTCTCACTTTGGTTCCTTAGTAAACGGACACATACGGTATTTAACAAGGAAACGAAGAACGACCACGAATC 6.36123418
TGCTTCCAAAACTCCTATGTCATGGTCAAAGGTGATAAATCACCTTACGACGGAGACTTAACGTACTGGAGCGAACGCAATAGCAAGCTCTATGACGGGGAAACCTCTAAAACTATTAAAAAGCAAAACCATACGTGTGGATACTGTGGCTTAAAGTGTACAAGTGAAGAACGAGTACACTTACACCATATAGATGGAAACCACA 11.39756201
AAAACAGGGAATATTCCGAGCATTAAAGGCAGGAATAAACCCAGAATTTCCCGAACAAGGAACACCTCAAGGTGGTGTGGTAAGTCCATTATTAGCAAACATCGCCTTAAATGGTATCGAGGATTTACACCAATACCACGACTGCAATTATAAGAAAATAACACCAAGTACACCGGAAAGGAACATCAAGAAAGCTTGTGTAAGATACGCCGATGATATGGTGTTCTTCTTAAGACCAGAAGACGATGCAGAAGAAATACTTGAGAAAATCAGCCAAT 33.70854848
GAGACTTAACTTACTGGAGCAAAAGAAACAGTAAATTATACGACGGCAGAACCTCAAAACTATTACAAAAGCAGCATCATACCTGTGGATACTGTGGATTAAGTATGCTCTCAGATGAGAGGGTACACCTACACCACATAGACAATAATCATAATAACTGGAAGGATTCAAACCTGATAGTAGTACATGAAAGTTGTCACGATTACATCCACATGAGCAAAAGAGGAAGTGAAATTTATCCAATTCCCTAGCGTGAAATCCCGTAATTAGGTGATAGGGAACAAAGATAGAAGGTAACTAACCCCTAAGAACATCGGAAGCTGGATGAAATGAAAG 24.23109402
GCGAAGCTTGCCAAGCAACTGATGTAATCATCTACACCCCGCTAACAAACTGGGCGTATCACATGGTAGACACGAATATTTATAAGTTGGCAACTAAATTATTAAACTAAGAGTCTTATTCATTATGTAATTCACTTTGC 14.1795265
CTGGTGCAAGAATTCCTCTTACCTTTTTGGCTGATTAATTAGCTTTACTTATGTTATGAGTCCAAATGAGCTATTCAATACCAGTATTTAAAAGCACAGTTTGGAGGCAAAAAGTTACTGGCACAGCTATTCATCATAGAGTGATCGCCAAACTTTTCGGTCTACTGAATGTACGTTAGTGGGGTACAGCCGGGATAAGCTGTCGTATAGCTAGATTGTATGATATGACATTAAACA 69.45726975
GTTCAACGCTGCGTTATTGTCTCTCAAATTGTAACCACTACAACCAGGACAAGCCCATTCACGTAACTTTAAATCTTTGACCAAAGGGTTGATAAAACCACAATGATTACAAGTCTGAGAGGAGGGATAAAACGTACCAACCTTCTGCACAATTCTGTCATGCCAAAAAGCTTTATATTCAAGCATGGCAACG 20.67189117
TTGGTAATTCTTTCGTAGCTACGAGCCAGCTTGATTTTCGCTTTGACTCGATTAGTACTGCCTTTTACACTGCGGGATAATTTTTTATGTGCTTTACGTAATTTACGAGTTTGAGTCCGGTAATATTTGGGATTATCTACGACTTCCCCATTACTAGTAACGAGATAAGACTTTATGCCTAAGTCTATGCCAATATTTTGACTAACTTGGGGATGTTTC 11.42331272
CGATTGATACCCAAATAAAGCATGAAGGTAGAGCATGAGAATTGCATTTGCCCAAGCTTGTTATCAGTGTAACGACCGCGTGCTGAAGTTGGTAACAGATGACGAACAGCATAGGCAAAGTCAGCATTAATCACTACTGTATCAAACTGATGGCGAGAAGCATCAGCCAGTTCCAAACCACGAACTTGCCCTTGTTCAATCCAGATTTGGTGAACAG 44.08896529
CCAAAAGCCCCTAAATGCCCAAATTACAAATGTTCATTTGACGATGGGAAATCCTACTAATGCTAACACTAGCTTTAGTAATTTCTTACTAAGTAAGGTTCAGTACGCAACCTCTCATAACTGTTTCCGAGGAACACCAAATTGGGTGAGTTGGCAGTTAAACCAATCATGGCTCGGAAGTGCGCCTCGTCAAGATGATTTTCGTGCAGATACTACCTTACCTTCTGGCTGCTATCGAGTCACATCTTCTGATTACACTGGTAGTGGATTTGATAGAGGACAC 39.0613087
CATCATTTCAGGTGCCTATGGCACTGGTGGTACAGGGTCGAATCAGAACAACACTACTACAATTGCTGGTGGTAAAGTTACTGTTCCAGCCCGAACCTATAAGGTGATTGTGGTTTTAGATAGACCAGGTTTGGGAGTGAGTGGTGTCACTACTAATACAAGGGTAATTGCAGTTGATATACCCAATACGCAAGGTGTGAGAAACGCGGATTGGAGAAATTATCGAATTAGCGTTGACACTCTAGAAGCAAGACTTAGGAATGCAACAGGAACTACCTATGATTTTCTTTCT 93.15018774
CAGCTTTATTGAACCAAACTCAATTGCTTCCCGTACTCTTGCTCCAAACACTCCACCTTGGGCCTAAAGAAACACACGCAGAAGCGATCGCCCACCACCCGATCAATCCCATGCGGCTGTTTGTTGTCAAAAGCAATACAATCGCCTTCCTCCAACCAATATGATTCCATCCGCCGAGTATCTTGGCAACCGGAGATTGAGAACTTCGCCCTACCCATAATGTTGATTTGCGCTGCTCCTGATGTGTAAGCCGGAGAATCCCGATGTACTTTAATCCTTTGGTAAGATGCAAATA 64.41704758
TACAGTCCAAAAAATAGCCTGCATAATCACACCTGGTTTATGCTTTTGACTTAATTATAGCTAAACCAAACTCAATTGCTTCCTGTACTCTTGCTCCAAATACTCCGCCTTGAGCCTAAAGAAGCACACACACCAGCGATCGCCCACCACCCGATCAATCCCATGCGGCTGCTTGTTGTCAAAAGCAATACAATCGCCCTCCTCCAGCCAATATGATTCCATCCGCCGCACATCCTGGCAACCAGAGATTGAGAACTTCGCTCGACCCATGATATTAATTTGCGCTGCTCCTGAAGCATAA 58.50203362
TGTTGACTGTTGAATAAGCCATTGGGGTAGGCAATGATGAAGATTCGTTTCCTTTCGTGCGGCAAGCCGACTTGTTGTGCAGAGATACAGTTCCATCCGCCCATGTACCCGATGCTGTCAAGGTCACAGATGATTTGGTCAAGTCCGCGATAGAGTAAACCTGTGGGGTTCTCGATGAGAGCGATCGCGGGTCTG 25.9918648
CTGTACCTCGGTTTTTTTCAAGCCGCTTGTTGATGTAAATAGGCATACATTTGGGGATGATTTGTTTCTAACCAGCTCAACCAGTTAGTCGTCGCGCCTGTGTGTTGTTGGTCATATCGGGTAGTGAATTGTGCGATCGCGCTTTCCCTTCCGACTCGATCTACAAGGTCTAGCAATTCGTT 11.87101118
CCCGCAATAAGCACATTCTCTATTCCACTTTTCAAGGAAATACTGTCGAGTTTCAAAACCAGCCAAAGTGCCTTGCTGATACTCTTTACCTTGAATATCTGGGTGGCGAATTAACTGCGTGTCAAACTTTACTAATTCTTGACTAATGGCTGTGATTGGTGCAACTTTCCTTAACCTATTTACCCAAGTTTTGATATTTTCAACTCGACTTTGCAAGCTTG 83.80121303
CTATTAGCCCTAGTGCTGGGAACTGGCTACGCATTCCCAGGTAAGATGACCTTAAAATCGTAGTGCGCGAAACACTTAGTCGGGTAAACTCTGGGCTTTGAAAGCCCCCACTATATTGCTTTTAGCAATCCGTGGTGGGTAGTTTACAAACTTA 6.891246096
AACTGTGGCTGAAGTTCTGCAACAGAAAGCGTTTGAATTTGGTCGTCAAAGTAAGAGCGAACTTCTTCTGAGTTTTTGTCAGCCATCACCGTATAACGCCAGCCTGTACCGTACTCATAAGCCAAAAGAGTATCAGTAGCGTAGTATTGGATACCGATGATTAGCCCTTGCTTTGTGCGCTGTCCTAACGTGAATCTGGGGTATTCCCAGCCCTTGGGTATGCTAATAGTGGGTTGCATGATTTCGGTAAAGGTGAAGCTAA 89.51379111
TATCATCAAGTATCTAAGTTTGCAGATAAGCAGTTTCCTGATTCGAGTACCCACAGCAGAATCGGGGATTGGAAAGTGACTAGAGTTGAAGAATATACCCCAGAAATTCCCAATCATGATTTTGAGGTTGTGGTTATTTGTTACTGCCAATACTCCCCAGTAACTACTCCACTAGAGCCAATGCCAGAAATTCAGGTTTCCCAAGAGGAACTACAGGAAGTTTAGCGGAATTTGGGCAACAAGCGAACACTGATAAAAATTTTGTAAGCATGA 78.91532111
ATAAGAGATTTTCACAATTGCGATGATCGCTGTTGCTGTTAAGCAAATTCTAGTCATCCGCCATCCACGAGATTTTGAGCAAGCCCCTAAGTCCAGTATTTCATAAATTTGTAACCAATTGAGTCTGCCTTGAGCAAAGGTTACAGCGATTCTATCAGGGAGCAGGTCAAACTTGTCAGTTCAGGTG 15.89127573
ACAAGATCAGACCGCCCAAACAAGCTGCTGTCCGCCAGCTACACTATCCAGATGTTTCAAGCTGAGTGGTTGAAGCTGTGAAAGTTTCTTGTCTTGGGCTTTTTGGGCTGTCTTGGTTTTTGTCTGTTGTTTATTGGTCATTGCTTTTCACCCACTTTTATTTAATTG 26.20221029
CGATATCGGGACAGGTGCGATCGCTAAATGTTAAAGATGGACAATGTGTAGCCAAGGATGATATTCTCGCCACCATCGATCCCTCTCAACAAAAACAGCAATTACAGCAACAACGGGATAAACTAGCTCAATT 17.912583
GCCAATGATAACATTCGCACAGGTGATGGTAATGACACCATTGTTGGTGGTGCAGGTAATGACAGCATAAGTGCAGGGAATGGGAACAACAACATCAC 5.81286314
CATCTTTGGGAACTCAGAGAACAGCGACTTTAACTATTGTTGATAACGATGCTCGTCCTGGTGCGATCGCCTTTAGTGCAGCTAACTACAGTGTTAATGAAAATGGTACACCTGTCACAGCAATCACTATTAACCGGACTGGTGGTAGTGATGGTGCAGTTAGTGTTACCTTAACTCCTAGTAACGGTACAGCAGTAGCACCTAGTGACTTTAATAGCAACCCAATTATAGTTAACTTTGCTAAC 50.73674219
ATGGAGCAACCATTGGCGCACAAAATACTGCCGTATTAACAATTATTGATAACGATCCTTTGCCTGGAACTCTTGCGTTCAGTAGTTCCAGCTACAGCACCAATGAAGACGGTACACCTGTTGCGGCTGTGACTATTACCCGGACAGGCGGTGCAAGCGGCGCAGTTAGCGTTACTCTGACACCTACTAATGGAAGTG 29.71298497
CCTTTTTTAATCAAAAATATGCAATTTTTCCGAAACAGGGTTTAGGAACTCTAGGAACCAGTAATGATGTTTACGCTGGAATTTCTATTGGTACAAATGGAGTAACTATCTCTGAACACACACTTAACTATATGCCATCTGTATTAGTGTATAACACTGCATTGTCAGGATGGAATCATATAGCATTAGTTTATGAGAATAAAACTCCAAAATT 90.50757167
TTAATTGCTAGCACTGACACTGATAATACAGTGAATGAAGGCGTAGGAATTGGGGAGAATAACAATACCACAATTACGCCTGTGACAGTGACTCCAGCTTATCGGGCAATTGTTTCCACAGATACCGAGACAGCTTTAGCTGGAAATTCTGTAATTTTGCGCGGACAAGCTATTAGTAATAGTGATAATTCTCCCGTGGCGTTTGAATTTGTCAAAGTCAGGGTAGAAAATAAAG 80.31758691
CGAGGGCAATGAATATTTCACCATTAAGCTGACTAACCCCACAGGTGGCGCGGTGATTGGAAATCAAAGTATAGCCACTGTTACAATTATTGATGACGAAGCCCCATCTGATATTACCGTCACCCCTCCCCTAGAAACTCCTAGTACCACAATTACCAGTGCAGTAGACTCTCAAG 23.95441375
AATAATTTCTGGGCAGATAGTCGCTTTGCTAACATCAAAGGTAATGATTTCACAAGCGTCATTATTGATACTGGGATTGATTTAAATCATCCCTTCTTCGGGGCTGATACTGACAATAACGGCATTGCTGATAAAATCGTCTATCAATATGACTTTGCTGATAACGATGCAGACGCAAGTGACAGAAACAATCA 19.57664604
CGACGGTGATAATGAAAATGATAATGTTACGAACACAGGATTTAACTACCCCCGCGTAGACTTATTGAAACTTGCGGAAGCTATTCTTAGCCTCACTGGTACAACTCCTAACCCCGATCCTGTCAATCCAGGTAACAACAATAACAACAACGGTACTACCACATCAGACAATACCATTAACCAAGTCCACACTGTCAACCTCGCCGCCGGACAAGTCCGCACAGACGTTGATTTCGGTAATCAACAAATTATCACC 83.19958902
GGCATTCCGGGAAATGTTGATGTGGGTACACTGAATATTACAGTACAAGCGATCGACACCAGCAACGCGAGTATCAGTGATAGCTTTGTTCTCACTATTACTAACCTCATCAATAATATTGTTGGTACATCGGGTAACAATACCCTCGCTGGCACACCCAATAACGACAACATTCAAGGCTTAGGCGGTAATGACATTATCTTTGGGTTAGCTGGTAATGACACCCTCAATGGTGGTACAGGCTCAGATACCATGACTGGAGGGTTAGGCGATGACACTTACATCGTTGATAACAATGTCGATAAAGTAGTAGAAAACCTCAATGAAGGTATTGATACTGTTC 69.66254217
GTCAAAGCATCGTACCAAATACCGTTTTTTGCGTAGATGGAGGCTTGTTCCTGTGGTAACAATTGTTTAAATTCATCACGTAATGCGGCATCCAAATTCACACGTTGCACCCAGCCTTCTACATAATCCAATGTCGCTGATT 16.90232949
AGGGAACATAGAACCAAAAGCTGGGCTGTTCTACACTAGTTAAGCCCCACACCTGAGTAATTGTGGTTTCTCCTGCACCCTGATTCAAGCGTTGTTCATAGCTTGGAACAAGAGCGGTTAAAGGCAAATCCAGTGCAGTACAATCTCCTCGTGAGGCTCCTTCGCCTCGGTTCCCTGGTAAACCGCGATCGGGTGGTGGCGGTGGTGGGTTAAAGCG 12.42012814
AACTTTGGGGTGCATTATTAACACATGGCGGCTGGCGACAAGGGTTGTACGAACGACGACAAGGGCTGCCTGAACAGTGGTCAATTCAGGAATGGTTGCAAGCAGGAGTATCGAATTTAGCACAGCAGCTTGGTTGGGGAATGACTAGATTGCAATTAGCTGCTCGTGGTCTGCGA 14.12402064
AGAGAAATTTTACGGTTTTGAGTACAAAATTTATTCTGACTTATGATGTCATGTTCGGCTCAATACAAGTCAATACGGTGTTCAGCCGGGATACTTGCAAGTGCGATTTGTTCTGGAGAAACCCAATACACTTCAAAATGAGCAACAAAACCCTGATGTAGAGACGCGATTTATCGCGTCT 59.2087911
GATATAGGCAGTAGTAATAACCTTTACGTGCCATTAATTCTTCATGAGTGCCTTGTTCTACTACAGCACCTTGTTCCATCATGAGAATGATGTTGGCATGGCGAATTGTGCTGAGGCGATGTGTGATGAAAAATACTGTTTTTCCTTGAAATGCTGTTGCCAGATTACGGCCAACTTGGGCTTCGGCGTTGTAGTCTAAGGCGCTGGTGGCTTCA 90.45008485
CTACCTTTGCGGCGGCAATAATTTCTTCATCACTGGCTTCGGAACTTCCCAAGGCAATGTTTTCCCGCACTGTCCCGTCAAATAATAAGGTATCTTGTAACACCATTCCAATTTGACGGCGTAAGGAATAAAGTTCAACCTTGGCGATATCATAGCCATCAATCAAGATTTTGCCAGATTTGGGTTCGTAAAGGCGCGGTAGTAATTTTAGCAATGTACTTTTACC 247.8329454
TGACTGTCTGCATCCCTCCCATCACTTCTACTAAGTAAGATTGAGTTTCAGCATTGCGTTCGGCTTTTTCTTGTAATTGTCGCCGCATTAAAGGCGATACTATCAAGTTAAGCAAGCCAAACAAGGGTACTGTTACCAATGCTACTAAGGTGAGAATTGGGCTATAAAATGCCATAACCACAATGTAGACTACAGAAAACACTGCATCCATCACCACAGTTAGGGCTGTACCAGTCAAAAAAGAACGGATATTTTCT 256.8320818
ATATTTGTAGAAATATTTAATCCGTGTAGTTATAAAATTTAGAAATGCAAGCGGCAAAGTTCAGGTAGAGTTCTGTACCGCTTGCAACATTTGCATACAGCGCTTCCGGCTATTATGCAATACAGTTTTTCTCCTGACCCGCTCCTATCAAAGCTTTCAGAGTTGAGGATGTACCTCATAGCTGCCGGAAGTGCTGTAATCTTGAAAGCATTGCTGTGCAATGCTTTCAAGTACCACT 80.30737221
GCATAATCTCTTTGTAAAATAGCGATAAAACAATCTACATAAGCAAGTGATAAATAACGAAGATATTCATCAAAGCTTTTCATTGCTTCTTCGGGATCATCGCTAATATTACGCAAGGAATTATCTGGCTCTAACTCCATCGTGTAGCCTACCATACGAAGATAAATTTTTCCTCTTTTCAAACGAGCAATATCTTCTTGAGGAATTGGAAATCTAATATTAGAAAGCTGATTAAGTTTCTCTAATAAGCTTGTTCTCATACCTACTCGCCTATTAGGTGG 266.3319248
TTCTAAAATCATGACTCGACTATGAGAAGCGGCGGTGAAATGTAATCTATCGAGTGCTTCTGTGGCAATATTGACAGCTGTATCAAATCCAATGGCGTGTTCAGTAATACCGATATCATTATCAATAGTTTTAGGGATACCGACTAAGTTGATAGCGCCTTGTTGTGCAAGGCGACGCAGAATTGCTAAACTACCATCCCCGCCAATGCCAATCA 47.38370908
AATGAACTTGTAGCAAGTATTTCTGCGGTTGCCTACTCTCAACACTTTGCCGTTATTGGCTTTTATATTGTCAAAGAGCAGTTCCGTGGTCGAGGTTTTGGCATGAAGATGTGGAGGACAGCAATGGCATACCTTGGTAGTGAGCGTAACATTAGCTTAGATGGAGTAATTGCTCAACAGGAGAATTACAAAAAATCAGGTTTTCAAATAGCCTACCGCCATATTCGTTATCAGG 209.1463855
TTCAACTCGAAGAATTTGTCAGCTTTCTTACCCGATTTCTGTAAGGCGATGAAGAAGCAATCCGATCTGCATCAACTCTCGAAACGGCAAAAAGATGCTCTGACTATGGCATTGTGGATGGGAAAGACATCTGATGCTAATGCTACAGTTTTTGCGGGTTGCATGAAGCCATTATTACTGCGTGCATGGGCGATTTATAAACGTCGTGAACAGCTATCAGCAGATAGCTTGTACCACTATCACTGTGACTTCAATCGCCGATTAAAGCGGTGTTTGGAATTACAGCAAGACAACAAGCGAGGTTCACAATCAAAGG 50.82712091
TCAACAAAATTAAATCGCCGTTGGCAAAATTTGTGGGAATACCTTGAGGTGGGAAAGCGGTTTGAAACACAAATGGAGGAGTAACACCCGCAACAAATGGAATGTCTACTCTAAGTCCTAATCCACCACCAGATGTAGCCCTAAATGAATTAGACAGAAAATCTGCCCGGTTGGGAGCAAAGGGGTTAAAGACTTTACCCAAGCT 12.93581541
TTGTTCAAAGCTCTAACTCTAGCTTTATTAGTATTGTTATCTGCATTTGTCCCCAGTGTTCTAGCAAACCAAAGCAGAAAGCCTGCCAATTTGGATGGCACAACAACACTCCAAAGCATACTGTCAAGCTCTCATCGCTCTGAGGAAAATCGGCAAAGAGACAAGTATCGTCACCCAACACAAACTTTGTCATTCTTTGGCTTACGACCAGACATGACAGTAGTTGAA 25.70570863
ATCCACAGCCCAGTTCCATCGTGGGGAAAAAACGGGCCACAGCACAGAAGTGTTCCTTTCTCTTGCTGCTCACGAAACCAAGTCAATTGGTCAGGATCATGTTCTGGGTGAGCTTTTTTATACTCATAATATTCGGGAACAGTTGCTACGACAACGGCGAAGAGTTTAGACATATGTTATTAGAT 27.21649586
ATTTGGTTTGACCAGGGGGCCGGGACGACTTGGTTGAATAAGCCATTGGAGTAGGCAATGATGAAGATTCGTTTCCTTTGGTGCGGCAAGCCGACTTGTTGTGCAGAGATACAGTTCCATTCGCCCATGTACCCGATGCTGTCAAGGTCACAGATGATTTGCTCAAGTCCGCGATAGAGTAAACCTGTGGGGTTCTC 15.39751116
CTTTCCTGGAGCCTGTACTTCGGTGACGCGCATCCCTTGAGGTAAGGGTAAAACGGCGCGTGATTGTTTAGTCAGAACTTGTTCGTCAGATGAAGTATCCAAACCGTGTTGTCGGCGTGTGTTGATGTTAACTAC 8.125250557
TCCTTGGGACACCGTATATTGATATGTTGTGCCTGGTTGCAAACCACGCACATCTACCGTGTGTTTGTAGTTATACTCACTGAGTAACCAAGAACCTGGAGATAGACCAGCAATATTTTGATTCTTCTCAGCGTTAGTATATGCAAGTACCGACTGGTATGTAGGTGTGCTAGTAAATGTGAGAGGATTGGTTAGACCAGTTCCAGTAATTGAGAGGATACCGGGTGCGTCTTTCTCTGTAAAC 74.11291598
TTCTTCTAAAAGATTGCCGTATGATATTTGATAGAGATAATTTTGTCGAACTTGCTCTAATCTTGTTTGCTCTGTTTCACTTAATACAGGCAAATCAGCCATCCATTCAGTGAAAAATTGATGATCATTACTTAGTGTTAATCCTAGTTTCTCCTCAACTTGTCGAAGGGTAGTAATTTTTTCTGCAATAGCTATGCTTGTGGTCATGTTGAAACTTTCATTACTCGC 50.45295721
GCCTGGGCTGCCTTTTTTAATACAGTAACTACTTGTTGCAGGTCGCTATCGTAACCAATGAATAGTTCGACACTACTACGGCGGATAGGTGCAGCTGTGTTATTAATGATCCGAGAAGTAAACACTTCGGCATTGGGA 5.904363224
AATTGCCTGTGACCATTGGATAGCTAACGTATCAACAGTTGTTAAATTGTCTTGAGCATCTGTTGTAGTTAGAGTAACAATTGGCACTCCAACATTAGTAATAACGCGTTCTTGCTTATCTTGAGAAGTTTCAATTTG 16.04040246
AGTCCCAGGAATGGTTCCCCTATTCCCATCGAAGGAGGTTTGTCGGCTTGTGCTGAAGCTGGCTTCGGTATAACTGATGTCAAATTTAAAGCTTTTGTTCAGCAGTTGCGCTCAATTACACCCCGCTCAGGAACTTTCTTAAATGTAGAACTTGCTGCAAGCTGTAGGCTCAACTAAAAATTTGGGTGCAAG 11.59957309
CTTTTGTGTCCATATTGCAGCTAAAATTGCCTCTCACACTCTACGTCTATTACTTCGCCGCCGTTTTGGTATCAATGTTCTCACTTTCCAATCTCAACCTAACTAGGAGAGCCGTGACGTACCTACAC 12.42534239
GGCGTAACATAATACGAGTCATAACAGCGTATATGGCAGCTTCACTCATTTCTGACAGATGCTCATAATCCTTACTAAGACGACGGTACTGGTTCAACCAACCGAATGTTCTTTCTACTACCCAACGCTTGGGAAAAACCTTCCATTCTTGTTCAGTACGCCGTATTACCTCAACAT 23.71477805

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
GTTGTTGGCGATAC
... ... asl7114 all7115
Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer
GTTGTAACAGTTAC
... ... all7195 all7196 all7194
Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer
GTTACGCTCGATAC
... ... all7332 all7331 alr7333 alr7334
Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer
GTTACGCTCGATAC
... ... alr7341 alr7342 asr7343
Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer
GTGTAGGGCAATAC
... ... all7084 all7085 alr7086
Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer
GTGTAACTTTAAAC
... ... all7244 all7245 asl7246 asr7247
Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer
GTGTAACTTACCTC
... ... all7091 alr7092 alr7093 alr7094
Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer
GTGCTTGCTGATAC
... ... alr7129 all7130
Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer
GTATTCTGCCGAAC
... ... all7377 alr7378
Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer
GTATTCGCTGATAC
... ... alr7025 all7026
Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer
GTATAGTATTTAAC
... ... all7348 alr7349 alr7350
Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer
GTAGCCTGTGATAC
... ... alr7242 alr7243
Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details PSSM site search (ECO:0005659) - not specified dimer
GTAACTGCTGTAAC
... ... all7303 alr7304 all7302 alr7305
activator monomer