(1) Demonstration of binding by EMSA and DNAse footprinting. (2) Demonstration of regulation by beta-gal reporter assay induced by cAMP.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.