Curation Information

Phosphate limitation induces catalase expression in Sinorhizobium meliloti, Pseudomonas aeruginosa and Agrobacterium tumefaciens.;Yuan ZC, Zaheer R, Finan TM;Molecular microbiology 2005 Nov; 58(3):877-94 [16238634]
PhoB [M4MSP4, view regulon]
Reported TF sp.
Sinorhizobium meliloti RCR2011
Reported site sp.
Sinorhizobium meliloti RCR2011
Created by
Matthew Coveyou
Curation notes

Experimental Process

A consensus search identified the putative binding site. Site expression was induced in PhoB variable strains via phosphate starvation, and GUS assay indicated expression dependence on PhoB. A Western blot of PhoB variable cell extracts confirmed a loss of expression in the absence of PhoB. EMSA identified strong binding to the putative site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... katA oxyR
Experimental technique details Alkaline phosphatase reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details GUS reporter gene assay (ECO:0005641) - Experimental technique details PSSM site search (ECO:0005659) - activator not specified