Curation Information

Publication
Interaction of transcriptional repressor ArgR with transcriptional regulator FarR at the argB promoter region in Corynebacterium glutamicum.;Lee SY, Park JM, Lee JH, Chang ST, Park JS, Kim YH, Min J;Applied and environmental microbiology 2011 Feb; 77(3):711-8 [21115700]
TF
ArgR [O85175, view regulon]
Reported TF sp.
Corynebacterium glutamicum ATCC 13032
Reported site sp.
Corynebacterium glutamicum ATCC 13032
Created by
Dinara Sagitova
Curation notes
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Experimental Process

Multiple sequence alignment identified identified a putative ArgR box within the argB promoter. EMSA with different length probes of the argB promoter identified the sequence region important for ArgR binding. RT-PCR confirmed that argB expression is regulated by ArgR. Site-directed mutagenesis in conjunction with EMSA further verified the functionality of the putative ArgR binding site.

Transcription Factor Binding Sites


TCCGCGTACAGCTCTTAAAAAGAAACA
TCCGCGTACAGCTCTTAAAAAGAAACA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TCCGCGTACAGCTCTTAAAAAGAAACA argB,
... ... argB argJ argD argF argR
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator not specified