Curation Information

Publication

MexZ-mediated regulation of mexXY multidrug efflux pump expression in Pseudomonas aeruginosa by binding on the mexZ-mexX intergenic DNA.;Matsuo Y, Eda S, Gotoh N, Yoshihara E, Nakae T;FEMS microbiology letters 2004 Sep 1; 238(1):23-8 [15336398]

TF

MexZ [G3XCU9, view regulon]

Reported TF sp.

Pseudomonas aeruginosa PAO1

Reported site sp.

Pseudomonas aeruginosa PAO1

Created by

Joe Sparenberg

Curation notes

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Experimental Process

EMSA was used to test physical interaction of MexZ with the mexXY promoter region and showed direct binding at the mexZ-mexZ intergenic region. EMSA was used on mexXY promoter fragments of different lengths to locate the binding sites and included an inverted repeat of 10-bp separated by 4-bp.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.