Curation Information

Publication

MexR repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa: identification of MexR binding sites in the mexA-mexR intergenic region.;Evans K, Adewoye L, Poole K;Journal of bacteriology 2001 Feb; 183(3):807-12 [11208776]

TF

MexR [P52003, view regulon]

Reported TF sp.

Pseudomonas aeruginosa K870

Reported site sp.

Pseudomonas aeruginosa K870

Created by

Matthew Coveyou

Curation notes

This curation needs to be edited next time Matt is in the lab.

Experimental Process

The P. aeruginosa mexAB-oprM operon was selected for investigation, and EMSA demonstrated specific binding of mexR to three sites upstream of the mexR gene, but none upstream of mexA. DNase I assay identified two protected regions, designated Site I and II, consistent with the EMSA. RACE and RT-PCR of Site II in wild-type and mutant strains both confirmed the down-regulation of mexAB-oprM upon mexR binding.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.