Curation Information

Publication

Role of MexZ and PA5471 in transcriptional regulation of mexXY in Pseudomonas aeruginosa.;Yamamoto M, Ueda A, Kudo M, Matsuo Y, Fukushima J, Nakae T, Kaneko T, Ishigatsubo Y;Microbiology (Reading, England) 2009 Oct; 155(Pt 10):3312-21 [19589837]

TF

MexZ [G3XCU9, view regulon]

Reported TF sp.

Pseudomonas aeruginosa PAO1

Reported site sp.

Pseudomonas aeruginosa PAO1

Created by

Joe Sparenberg

Curation notes

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Experimental Process

EMSA revealed that MexZ binds to a 20bp palindromic sequence to the promoter region of the mexXY operon. EMSA and Western Blot showed higher levels of MexX and MexZ in the presence of tetracycline. Plasmid-borne MexZ repressed drug-induced MexX production confirming that MexZ acts as a repressor of the mexXY operon. EMSA and in vitro transcription assays revealed the interaction between PA5471 and MexZ reduces MexZ DNA-binding ablility, leading to mexXY transcription.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.