Curation Information

Publication

Purification and characterization of CopR, a transcriptional activator protein that binds to a conserved domain (cop box) in copper-inducible promoters of Pseudomonas syringae.;Mills SD, Lim CK, Cooksey DA;Molecular & general genetics : MGG 1994 Aug 15; 244(4):341-51 [8078459]

TF

CopR [Q87Z28, view regulon]

Reported TF sp.

Pseudomonas syringae PT23.2

Reported site sp.

Pseudomonas syringae PT23.2

Created by

Grace Chandler

Curation notes

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Experimental Process

Site-directed mutagenesis combined with lacZ transcriptional fusion narrowed down the region of cop promoter necessary for copper inducible expression. EMSA determined that CopR binds specifically to the cop promoter. DNase I footprinting localized the CopR binding site.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.