Curation Information

Publication
CcpA ensures optimal metabolic fitness of Streptococcus pneumoniae.;Carvalho SM, Kloosterman TG, Kuipers OP, Neves AR;PloS one 2011; 6(10):e26707 [22039538]
TF
CcpA [A0A0H2ZQ65, view regulon]
Reported TF sp.
Streptococcus pneumoniae D39
Reported site sp.
Streptococcus pneumoniae D39
Created by
Elliot White
Curation notes
-

Experimental Process

Researchers used a consensus search of the CRE box (CcpA binding site) in the pneumoniae genome to compile a list of genes with CRE boxes in the promoter. They then cross-referenced that list with the results (trimmed to only significant results) of a DNA-array that compared wt to a CcpA deletion.

Transcription Factor Binding Sites


TTTGTAAGCTATTCCAAAC
ATTGACAACACTGCCAAAA
ATCGAAAACGATAGCATCA
ATTGTTACCGATCACATTC
TTTGTAAGCTATTCCAAAC
ATTGACAACACTGCCAAAA
ATCGAAAACGATAGCATCA
ATTGTTACCGATCACATTC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TTTGTAAGCTATTCCAAAC folA,
... ... folA SPD_1402 SPD_1397 engB clpX SPD_1400
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor dimer
ATTGACAACACTGCCAAAA pstS,
... ... pstS pstB phoU pstA pstC SPD_1908 pnpS
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor dimer
ATCGAAAACGATAGCATCA SPD_1026,
... ... SPD_1026 lpdA SPD_1027 acoA
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor dimer
ATTGTTACCGATCACATTC SPD_0098,
... ... SPD_0098 capD SPD_0097
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor dimer