Curation Information

Publication

Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR.;Terán W, Felipe A, Segura A, Rojas A, Ramos JL, Gallegos MT;Antimicrobial agents and chemotherapy 2003 Oct; 47(10):3067-72 [14506010]

TF

TtgR [Q9AIU0, view regulon]

Reported TF sp.

Pseudomonas putida DOT-T1E

Reported site sp.

Pseudomonas putida DOT-T1E

Created by

Joe Sparenberg

Curation notes

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Experimental Process

lacZ analysis showed that TtgR is a repressor of the ttgABC operon and the ttgR gene. EMSA showed that TtgR binds specifically to the ttgR-ttgABC intergenic region. DNase I footprinting assays identified the region bound by TtgR in the ttgR-ttgABC intergenic region.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.