Curation Information

Publication

Characterization of OxyR as a negative transcriptional regulator that represses catalase production in Corynebacterium diphtheriae.;Kim JS, Holmes RK;PloS one 2012; 7(3):e31709 [22438866]

TF

OxyR [H2GUE0, view regulon]

Reported TF sp.

Corynebacterium diphtheriae C7 (beta)

Reported site sp.

Corynebacterium diphtheriae C7 (beta)

Created by

Joe Sparenberg

Curation notes

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Experimental Process

lacZ reporter assay and qRT-PCR showed that OxyR represses the cat gene. EMSA performed with cat promoter fragments of various lengths and DNase I footprinting determined the location of the OxyR binding site.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.