Curation Information

Role of Pseudomonas aeruginosa dinB-encoded DNA polymerase IV in mutagenesis.;Sanders LH, Rockel A, Lu H, Wozniak DJ, Sutton MD;Journal of bacteriology 2006 Dec; 188(24):8573-85 [17041045]
LexA [P37452, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes
dinB locus tag, PA0923, was not shown by CollecTF search.

Experimental Process

qRT-PCR experiments showed that dinB expression increased 5.5-fold in lexA mutant compared to wild type P.aeruginosa PAO1. Western blotting experiments showed that DinB concentration increased in response to DNA damage. EMSA confirmed that LexA bound to dinB promoter region.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... PA0922
Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Visual sequence inspection (nan) - Experimental technique details Western blot (quantitative) expression analysis (ECO:0000279) - repressor not specified