qRT-PCR experiments showed that dinB expression increased 5.5-fold in lexA mutant compared to wild type P.aeruginosa PAO1. Western blotting experiments showed that DinB concentration increased in response to DNA damage. EMSA confirmed that LexA bound to dinB promoter region.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|