Curation Information

Publication

Two levels of cooperativeness in the binding of TodT to the tod operon promoter.;Lacal J, Guazzaroni ME, Gutiérrez-del-Arroyo P, Busch A, Vélez M, Krell T, Ramos JL;Journal of molecular biology 2008 Dec 31; 384(5):1037-47 [18950641]

TF

TodT [I7CA98, view regulon]

Reported TF sp.

Pseudomonas putida DOT-T1E

Reported site sp.

Pseudomonas putida DOT-T1E

Created by

Dinara Sagitova

Curation notes

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Experimental Process

EMSA with a 352-bp todX promoter fragment showed that up to four different complexes were formed. A series of ITC assays further verified binding of TodT to todX promoter region. Beta-galactosidase assays showed that mutagenesis of both half-palindromes resulted in an almost 12-fold decrease in transcriptional activity.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.