Curation Information

Publication

BphS, a key transcriptional regulator of bph genes involved in polychlorinated biphenyl/biphenyl degradation in Pseudomonas sp. KKS102.;Ohtsubo Y, Delawary M, Kimbara K, Takagi M, Ohta A, Nagata Y;The Journal of biological chemistry 2001 Sep 28; 276(39):36146-54 [11459836]

TF

BphS [E7FJA6, view regulon]

Reported TF sp.

Pseudomonas sp. KKS102

Reported site sp.

Pseudomonas sp. KKS102

Created by

Dinara Sagitova

Curation notes

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Experimental Process

Northern blot analysis and LacZ reporter assays demonstrated that BphS negatively regulates bph operon expression. bphE transcription start site was identified by primer extension analysis. BphS binding region was identified by deletion analysis of the bphE promoter with lacZ reporter assays. EMSA confirmed BphS binding to this region. BphS binding sites were localized by DNase I footprinting assays.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.