Curation Information

Publication
BrlR from Pseudomonas aeruginosa is a c-di-GMP-responsive transcription factor.;Chambers JR, Liao J, Schurr MJ, Sauer K;Molecular microbiology 2014 May; 92(3):471-87 [24612375]
TF
BrlR [Q9HUT5, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes
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Experimental Process

brlR transcription start site was identified by 5'RACE. EMSA showed that BrlR specifically binds to its own promoter. ChIP-qPCR confirmed BrlR binding to its promoter in vivo. Site-directed mutagenesis of the putative BrlR binding site combined with EMSA showed that mutating one half of the palindromic sequence impaired BrlR binding. Promoter-lacZ fusion assays showed that brlR expression is enhanced by c-di-GMP. MEME motif discovery algorithm was used to identify the BrlR binding motif.

Transcription Factor Binding Sites


TGACCTTGCCCCAGGGGCAATCCGTAGT
TGACCTTGCCCCAGGGGCAATCCGTAGT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGACCTTGCCCCAGGGGCAATCCGTAGT PA4878,
... ... PA4878 PA4877
Experimental technique details Beta-gal reporter assay - Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator dimer