Curation Information

Publication
Time-resolved determination of the CcpA regulon of Lactococcus lactis subsp. cremoris MG1363.;Zomer AL, Buist G, Larsen R, Kok J, Kuipers OP;Journal of bacteriology 2007 Feb; 189(4):1366-81 [17028270]
TF
CcpA [Q9CF33, view regulon]
Reported TF sp.
Lactococcus lactis subsp. cremoris MG1363
Reported site sp.
Lactococcus lactis subsp. cremoris MG1363
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

Comparison of the transcriptional profile of L.lactis MG1363 and its ccpA mutant by DNA microarray experiment identified differentially expressed genes. Consensus search in the L.lactis MG1363 genome identified the presence of CcpA binding sites in the promoters of the CcpA-regulated genes. ccpA-lacZ reporter assay identified that ccpA expression was 2.5-fold lower in the wild type than in L.lactis MG1363 ccpA mutant. EMSA confirmed that CcpA binds to the ccpA -pepQ intergenic region. Northern blot analysis confirmed that pepQ expression was significantly reduced in the ccpA mutant strain.

Transcription Factor Binding Sites


TGTAAACCTTTTCA
AGTAACCGTTTACA
AGAAATCGCTAACA
TGTAACAGTTTACA
AGTAACCGGTATTA
TGAAAGAGCATACA
TGACAGAGTTTTCA
TTAAAACGTTTGAA
ATAAAACGCATACA
ATAAAGCGATTTCT
AGTAAGCGTATTCA
TGTAAACCTTTTCA
TGTAAGAGATATCT
TGAAAACTTTTACA
TGTAAACGATTCCA
TGAAATCATTAGCA
TGTAAGCGCAAGCA
TGTGAACGCTTAAA
TGTAATGGATTTCA
TGAAAGTGATAACA
TGCACACGTTATCT
TGGTAGCGGTTATA
TGTAAAAGCTTACA
TGAAAACCTTTGCA
TGAAAACGTTATAA
AGAAACCGCTTTCT
TGAAAACGTTTTTA
ATAAACCGTTTTCA
TGAAATCGCTATCA
AGTAATCGTTATCT
TGAAACAGTTATCA
ATAAAGCGTTTACA
TGAAAAAGGTAACA
TGAAAGCGAATAAA
AGTTAGCGCTTACA
TATCAGCGTTAACA
TGCCATCGTATTCA
TGAAAACGCTTTTT
AATAAGCGCTTACA
TGAAAAAGGTAACA
TGTAAGTATTTTCA
AGAAAATGTTTTCA
TGCAAGCGATTTGA
TGATATCGCTTCCA
GGTAATCGTTTACT
TGAAAGCGCAAACA
AAAAAGCGTATTCA
TTAATACGATTACA
TGAAAGCGTTAAGA
TGTAAAGGCTTAAA
TTAAAGCGGTTAAA
TGAAAACTCTAACA
TAAATACGCTTTCA
TGAATACGAATACA
TGAAATCGTTCACA
TGTAAGCGTTGATA
TTTAAGCGTTCACA
TGATATCGATAACA
TTTAAACGTTTACC
AGAATGCGTTTACA
TGTCAGCATTTTCA
TGTAATCGGTTACA
TGAAAGCACTGCCA
TGAAAGTGCTTGCA
TGATAACGCTGACA
AGAAAGCGTTTTAT
TGCGAACGCATTCA
AGAAAGCGGACTCA
TAGAAGCGATAACA
GGCAAACGTTATCA
TGTATGCGTTTTAA
AGAAAGGGTTTTCT
AGAAAGCGCTATAA
TGTAAACCTTTTCA
AGTAACCGTTTACA
AGAAATCGCTAACA
TGTAACAGTTTACA
AGTAACCGGTATTA
TGAAAGAGCATACA
TGACAGAGTTTTCA
TTAAAACGTTTGAA
ATAAAACGCATACA
ATAAAGCGATTTCT
AGTAAGCGTATTCA
TGTAAACCTTTTCA
TGTAAGAGATATCT
TGAAAACTTTTACA
TGTAAACGATTCCA
TGAAATCATTAGCA
TGTAAGCGCAAGCA
TGTGAACGCTTAAA
TGTAATGGATTTCA
TGAAAGTGATAACA
TGCACACGTTATCT
TGGTAGCGGTTATA
TGTAAAAGCTTACA
TGAAAACCTTTGCA
TGAAAACGTTATAA
AGAAACCGCTTTCT
TGAAAACGTTTTTA
ATAAACCGTTTTCA
TGAAATCGCTATCA
AGTAATCGTTATCT
TGAAACAGTTATCA
ATAAAGCGTTTACA
TGAAAAAGGTAACA
TGAAAGCGAATAAA
AGTTAGCGCTTACA
TATCAGCGTTAACA
TGCCATCGTATTCA
TGAAAACGCTTTTT
AATAAGCGCTTACA
TGAAAAAGGTAACA
TGTAAGTATTTTCA
AGAAAATGTTTTCA
TGCAAGCGATTTGA
TGATATCGCTTCCA
GGTAATCGTTTACT
TGAAAGCGCAAACA
AAAAAGCGTATTCA
TTAATACGATTACA
TGAAAGCGTTAAGA
TGTAAAGGCTTAAA
TTAAAGCGGTTAAA
TGAAAACTCTAACA
TAAATACGCTTTCA
TGAATACGAATACA
TGAAATCGTTCACA
TGTAAGCGTTGATA
TTTAAGCGTTCACA
TGATATCGATAACA
TTTAAACGTTTACC
AGAATGCGTTTACA
TGTCAGCATTTTCA
TGTAATCGGTTACA
TGAAAGCACTGCCA
TGAAAGTGCTTGCA
TGATAACGCTGACA
AGAAAGCGTTTTAT
TGCGAACGCATTCA
AGAAAGCGGACTCA
TAGAAGCGATAACA
GGCAAACGTTATCA
TGTATGCGTTTTAA
AGAAAGGGTTTTCT
AGAAAGCGCTATAA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGTAAACCTTTTCA ccpA,
... ... ccpA pepQ trxB2
Experimental technique details Beta-gal reporter assay - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - repressor not specified
AGTAACCGTTTACA glpK,
... ... glpK glpD glpF2 rluC
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
AGAAATCGCTAACA llmg_1127,
... ... llmg_1127 llmg_1128 llmg_1129
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGTAACAGTTTACA noxB,
... ... noxB sdaB sdaA copR merP
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
AGTAACCGGTATTA cydA,
... ... cydA cydB
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGAAAGAGCATACA llmg_1915,
... ... llmg_1915 llmg_1916 llmg_1917
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGACAGAGTTTTCA llmg_1916,
... ... llmg_1916 llmg_1917
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TTAAAACGTTTGAA ackA1,
... ... ackA1 ackA2 llmg_2286 pyrH
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
ATAAAACGCATACA ackA2,
... ... ackA2 llmg_2286 pyrH
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
ATAAAGCGATTTCT adhE
... ... adhE
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
AGTAAGCGTATTCA
... ... llmg_tRNA_10
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGTAAACCTTTTCA pepQ,
... ... pepQ ccpA trxB2
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGTAAGAGATATCT busAA,
... ... busAA busAB
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAAACTTTTACA arcB,
... ... arcB arcD1 arcC1 arcC2
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGTAAACGATTCCA arcA
... ... arcA
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAATCATTAGCA trpE,
... ... trpE trpG trpD
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGTAAGCGCAAGCA gntP
... ... gntP
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGTGAACGCTTAAA udk,
... ... udk llmg_0761 llmg_tRNA_35
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGTAATGGATTTCA purQ,
... ... purQ purL purF
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAAGTGATAACA gmk,
... ... gmk rpoZ
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGCACACGTTATCT
... ... purA
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGGTAGCGGTTATA mtlA,
... ... mtlA mtlR mtlF mtlD
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGTAAAAGCTTACA mtlD
... ... mtlD
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAAACCTTTGCA glgB
... ... glgB
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAAACGTTATAA ptcB,
... ... ptcB ptcA llmg_0439
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
AGAAACCGCTTTCT ptcC,
... ... ptcC celA
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAAACGTTTTTA llmg_0453,
... ... llmg_0453 treR llmg_0454 trePP
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
ATAAACCGTTTTCA malE,
... ... malE malF malG dexC
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAATCGCTATCA arb
... ... arb
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
AGTAATCGTTATCT rpe2,
... ... rpe2 llmg_0956 rpiB llmg_0959
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGAAACAGTTATCA
... ... llmg_0959 llmg_0960
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
ATAAAGCGTTTACA llmg_1468,
... ... llmg_1468 llmg_1467
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAAAAGGTAACA fruC,
... ... fruC fruR fruA
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGAAAGCGAATAAA pmi,
... ... pmi llmg_1790
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
AGTTAGCGCTTACA chiC,
... ... chiC chb
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TATCAGCGTTAACA galK,
... ... galK galT galE llmg_2232
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGCCATCGTATTCA galP,
... ... galP galM galK
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAAACGCTTTTT llmg_2431,
... ... llmg_2431 rpsB tsf
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
AATAAGCGCTTACA pgi,
... ... pgi llmg_2449
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGAAAAAGGTAACA fruR,
... ... fruR fruC fruA
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGTAAGTATTTTCA dltD,
... ... dltD dltB dltC
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
AGAAAATGTTTTCA rpsA
... ... rpsA
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGCAAGCGATTTGA
... ... mnmA rpsA llmg_1726 llmg_1727 llmg_1728
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGATATCGCTTCCA
... ... ccpA pepQ trxB2
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
GGTAATCGTTTACT llmg_0956,
... ... llmg_0956 rpe2 rpiB llmg_0959
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGAAAGCGCAAACA llmg_2218
... ... llmg_2218
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
AAAAAGCGTATTCA llrB,
... ... llrB llmg_1069 kinB llmg_1066 llmg_1070 llmg_1071 tnp1077 llmg_1072 llmg_1073
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TTAATACGATTACA llmg_2320,
... ... llmg_2320 snf
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAAGCGTTAAGA clpP,
... ... clpP llmg_0639
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGTAAAGGCTTAAA phnD,
... ... phnD llmg_0311 phnC phnB llmg_0315 cpdC llmg_0317 tpx
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TTAAAGCGGTTAAA
... ... fhuD fhuR
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGAAAACTCTAACA
... ... llmg_0661 tnp905
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TAAATACGCTTTCA fdhC
... ... fdhC
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAATACGAATACA llmg_0185,
... ... llmg_0185 llmg_0186 celB llmg_0188 llmg_0189 bglS dut llmg_0192 llmg_0193
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAATCGTTCACA llmg_0194
... ... llmg_0194
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGTAAGCGTTGATA pgmA
... ... pgmA
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TTTAAGCGTTCACA llmg_0761,
... ... llmg_0761 llmg_tRNA_35 udk
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGATATCGATAACA llmg_0995
... ... llmg_0995
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TTTAAACGTTTACC llmg_1920,
... ... llmg_1920 llmg_1919 llmg_1918 rpsT
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
AGAATGCGTTTACA adhA,
... ... adhA llmg_1990 recQ
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGTCAGCATTTTCA
... ... llmg_1936 llmg_pseudo_59 aroC llmg_1933
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGTAATCGGTTACA cstA
... ... cstA
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAAGCACTGCCA
... ... llmg_0989
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGAAAGTGCTTGCA llmg_0183,
... ... llmg_0183 llmg_0184 llmg_0185
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGATAACGCTGACA llmg_0311,
... ... llmg_0311 phnD phnC phnB llmg_0315 cpdC llmg_0317 tpx
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
AGAAAGCGTTTTAT llmg_1090,
... ... llmg_1090 llmg_1091
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TGCGAACGCATTCA
... ... llmg_1092 llmg_1093 llmg_1094 llmg_1095 llmg_1096
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
AGAAAGCGGACTCA
... ... llmg_1096
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
TAGAAGCGATAACA
... ... llmg_1388 llmg_1387 llmg_1386 llmg_1385 llmg_1384 traD llmg_1382 llmg_1381 llmg_1379 llmg_1378 llmg_1377 ltrC ltrD
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
GGCAAACGTTATCA llmg_1750,
... ... llmg_1750 llmg_1749 llmg_1748
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - repressor not specified
TGTATGCGTTTTAA ps412,
... ... ps412 ps413 ps414 ps415
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
AGAAAGGGTTTTCT llmg_2211
... ... llmg_2211
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified
AGAAAGCGCTATAA llmg_2286,
... ... llmg_2286 pyrH
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - activator not specified