Curation Information

Publication

Coactivation of Vibrio vulnificus putAP operon by cAMP receptor protein and PutR through cooperative binding to overlapping sites.;Lee JH, Choi SH;Molecular microbiology 2006 Apr; 60(2):513-24 [16573699]

TF

PutR [Q7MBU9, view regulon]

Reported TF sp.

Vibrio vulnificus ATCC 29307

Reported site sp.

Vibrio vulnificus ATCC 29307

Created by

Dinara Sagitova

Curation notes

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Experimental Process

Primer extension assay performed with the wild-type and putR mutant strains showed that putAP expression was decreased in the putR mutant. EMSA showed that PutR binds to the putAP promoter. DNase I footprinting assay revealed that PutR protected two regions in the putAP promoter which extended from -97 to -80 and from -139 to -123. put–luxAB transcriptional fusions with a wild-type PutR binding site and with a promoter in which the binding site was deleted confirmed the PutR activated the expression of the putAP operon.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.