Curation Information

Publication

HlyU acts as an H-NS antirepressor in the regulation of the RTX toxin gene essential for the virulence of the human pathogen Vibrio vulnificus CMCP6.;Liu M, Naka H, Crosa JH;Molecular microbiology 2009 Apr; 72(2):491-505 [19320834]

TF

H-NS [A0A087I6S4, view regulon]

Reported TF sp.

Vibrio vulnificus CMCP6

Reported site sp.

Vibrio vulnificus CMCP6

Created by

Dinara Sagitova

Curation notes

-

Experimental Process

Comparison of the rtxA1-lacZ expression levels in the hns+ and hns- strains revealed that H-NS negatively regulates rtxA1 expression. EMSA determined that up to 0.7 uM of H-NS had to be added before binding was observed. DNase I footprinting verified this result by showing that binding was not observed until 0.8 uM of H-NS was added.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.