Curation Information

Publication
The iron-responsive Fur regulon in Yersinia pestis.;Gao H, Zhou D, Li Y, Guo Z, Han Y, Song Y, Zhai J, Du Z, Wang X, Lu J, Yang R;Journal of bacteriology 2008 Apr; 190(8):3063-75 [18281395]
TF
Fur [P33086, view regulon]
Reported TF sp.
Yersinia pestis CO92
Reported site sp.
Yersinia pestis CO92
Created by
Elliot White
Curation notes
No revision required

Experimental Process

Researchers used a dna-array to generate a list of candidate genes for Fur regulation, then double-checked that list with qRT-PCR, and confirmed binding with EMSA.

Transcription Factor Binding Sites


AATAAGAATTATTATCATT
AATGATCATAATTTCAAAT
CATGATAATCATTCACATT
AATAAGAATTATTATCATT
AATGATCATAATTTCAAAT
CATGATAATCATTCACATT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
AATAAGAATTATTATCATT napF,
... ... napF napD napA narP YPO3042
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator dimer
AATGATCATAATTTCAAAT katA,
... ... katA YPO1208
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator dimer
CATGATAATCATTCACATT ftn,
... ... ftn holE
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator dimer