Curation Information

Publication

The glycolytic genes pfk and pyk from Lactobacillus casei are induced by sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system and repressed by CcpA.;Viana R, Pérez-Martínez G, Deutscher J, Monedero V;Archives of microbiology 2005 Sep; 183(6):385-93 [16075200]

TF

CcpA [Q03AX7, view regulon]

Reported TF sp.

Lactobacillus casei BL23

Reported site sp.

Lactobacillus casei BL23

Created by

Dinara Sagitova

Curation notes

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Experimental Process

Northern blot analysis with mRNA obtained from the wild-type strain BL23 and ccpA mutant strain showed that pfk-pyk expression is repressed by CcpA. EMSA confirmed that CcpA bound to the pfk-pyk intergenic region. DNAse I footprinting showed that CcpA protected a region containing two degenerate CcpA motifs.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.