Curation Information

Publication: Catabolite repression in Lactobacillus casei ATCC 393 is mediated by CcpA.;Monedero V, Gosalbes MJ, Pérez-Martínez G;Journal of bacteriology 1997 Nov; 179(21):6657-64 [9352913]
TF: CcpA [Q03AX7, view regulon]
Reported TF sp.: Lactobacillus casei ATCC 393
Reported site sp.: Lactobacillus casei ATCC 393
Created by: Dinara Sagitova
Curation notes: -

Experimental Process

Visual inspection of the lacTEGF promoter identified a putative CcpA binding site with one mismatch to the CcpA consensus sequence. GUS reporter assays in the wild-type and ccpA mutant strains showed that repression by CcpA was abolished in the ccpA mutant strain.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

ATAAAACGTTTACA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type
ATAAAACGTTTACA	lact, lacE, lacG, lacF,		Experimental technique details GUS reporter gene assay (ECO:0005641) GUS reporter gene assay - ECO:0005641 This is a reporter assay technique, used typically to measure expression of a target gene. A promoter of interest is investigated by fusioning it with the reporter gene, which is then monitored. In GUS, the reporter is the β-glucuronidase enzyme from Escherichia coli, capable of transforming non-fluorescent substates into fluorescent, thereby allowing detection. - Experimental technique details Visual sequence inspection (nan) Visual sequence inspection - nan Visual inspection of promoter region to identify putative binding sites based on previous knowledge -	repressor	not specified