Curation Information

Publication

Transcriptional regulatory cascade for elastase production in Vibrio vulnificus: LuxO activates luxT expression and LuxT represses smcR expression.;Roh JB, Lee MA, Lee HJ, Kim SM, Cho Y, Kim YJ, Seok YJ, Park SJ, Lee KH;The Journal of biological chemistry 2006 Nov 17; 281(46):34775-84 [16971386]

TF

LuxT [Q7MFA2, view regulon]

Reported TF sp.

Vibrio vulnificus MO6-24/O

Reported site sp.

Vibrio vulnificus MO6-24/O

Created by

Erill Lab

Curation notes

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Experimental Process

LuxT was identified as binding the smcR promoter through SDS-PAGE. EMSAs confirmed that it binds specifically and DNAse footprint identified a protected region with sequence AGTGCAATACGCTATTTACTATCACA. A luxT- mutant was shown to induce smcR expression through Western blot and luciferase reporter.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.