sinR-gfp fusion assays in the wild-type and expR mutant strains revealed that sinR expression is reduced by the presence of AHL-activated ExpR. EMSA confirmed that ExpR bound to the sinR promoter region. By visual inspection of the sinR promoter, the researchers identified a 20-bp region homologous to the previously identified ExpR binding sites. The putative ExpR binding site was confirming by performing EMSA with a 34-bp fragment containing the predicted 20-bp site.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|