Curation Information

Novel Sinorhizobium meliloti quorum sensing positive and negative regulatory feedback mechanisms respond to phosphate availability.;McIntosh M, Meyer S, Becker A;Molecular microbiology 2009 Dec; 74(5):1238-56 [19889097]
ExpR [Q92L12, view regulon]
Reported TF sp.
Sinorhizobium meliloti 2011
Reported site sp.
Sinorhizobium meliloti 2011
Created by
Dinara Sagitova
Curation notes

Experimental Process

sinR-gfp fusion assays in the wild-type and expR mutant strains revealed that sinR expression is reduced by the presence of AHL-activated ExpR. EMSA confirmed that ExpR bound to the sinR promoter region. By visual inspection of the sinR promoter, the researchers identified a 20-bp region homologous to the previously identified ExpR binding sites. The putative ExpR binding site was confirming by performing EMSA with a 34-bp fragment containing the predicted 20-bp site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... sinR
Experimental technique details EMSA (ECO:0001807) - Experimental technique details GFP Promoter Fusion (ECO:0005636) - Experimental technique details Visual sequence inspection (nan) - repressor not specified