EMSA experiments identified that HapR binds to qstR promoter region from -150 to -102 bp. Visual inspection of this region identified a sequence resembling HapR binding motif 2. Site-directed mutagenesis was used to alter two conserved bases within this motif which resulted in the loss of binding by HapR.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
||not specified||not specified|