Curation Information

Publication
A transcriptional regulator linking quorum sensing and chitin induction to render Vibrio cholerae naturally transformable.;Lo Scrudato M, Blokesch M;Nucleic acids research 2013 Apr 1; 41(6):3644-58 [23382174]
TF
HapR [A0A0H3Q915, view regulon]
Reported TF sp.
Vibrio cholerae A1552
Reported site sp.
Vibrio cholerae A1552
Created by
Dinara Sagitova
Curation notes
Not ready for NCBI submission (find a better TF accession #)

Experimental Process

EMSA experiments identified that HapR binds to qstR promoter region from -150 to -102 bp. Visual inspection of this region identified a sequence resembling HapR binding motif 2. Site-directed mutagenesis was used to alter two conserved bases within this motif which resulted in the loss of binding by HapR.

Transcription Factor Binding Sites


TCACTGATAAACTTATCGGT
TCACTGATAAACTTATCGGT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TCACTGATAAACTTATCGGT
... ... VC0396 VC0397 VC0398 VC0399 VC0400 VC0401 VC0402 VC0403 VC0404 VC0405 VC0406 VC0407 VC0408 VC0409 VC0410 VC0411 VC0412 VC0413 VC0414
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - not specified not specified